植物内生真菌 Penicillium tropicum发酵产物中化学成分及其抑菌活性研究  

Antibacterial secondary metabolites from endophytic fungus Penicillium tropicum

作　　者：吕妍 贾少杰 戴好富 解修超[1] 曾艳波[2,3] LYU Yan;JIA Shao-jie;DAI Hao-fu;XIE Xiu-chao;ZENG Yan-bo(School of Bioscience and Engineering,Shaanxi University of Technology,Hanzhong 723000,China;Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources,Haikou 571101,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]海南省海洋生物资源功能性成分研究与利用重点实验室,海南海口571101 [3]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《陕西理工大学学报（自然科学版）》2020年第2期76-80,共5页Journal of Shaanxi University of Technology:Natural Science Edition

基　　金：海南省重点研发计划项目(ZDYF2017101);农业农村部财政专项资助项目(NFZX2018);国家自然科学基金资助项目(41776093);中国热带农业科学院基本科研业务费专项基金资助项目(1630052016011,1630052019011)。

摘　　要：对分离自植物Sapium ellipticum的新鲜叶片内生真菌Penicillium tropicum的发酵产物化学成分进行研究。对该菌株进行大米固体发酵培养,并用乙酸乙酯提取发酵产物;用多种柱色谱技术分离纯化出单体化合物;用多种光谱技术对单体化合物的结构进行了鉴定;并用微量稀释法测试了化合物抑菌活性。结论是,从该菌株发酵产物中分离得到5个化合物,其结构分别鉴定为epi-10,23-dihydro-24,25-dehydroaflavinine(1)、Penitropone(2)、(3 S)-3,5-dimethyl-8-methoxy-3,4-dihydro-1 H-isochromen-6-ol(3)、(-)-massoialactone(4)和(3 R,5 R)-3-O-β-D-mannosyl-3,5-dihydroxydecanoic acid(5)。抑菌活性测试结果表明化合物(1)对金黄色葡萄球菌USA300具有抑制活性,MIC为2.0μg·mL-1。The chemical constituents of endophytic fungus Penicillium tropicum isolated from the plant Sapium ellipticum were studied.The strain is cultured on rice solid medium.The ethyl acetate extracts were isolated by column chromatography.The isolated compounds were identified by their spectroscopic data.The antibacterial activity of the compounds were tested using microdilution method.Conclusion is that five compounds have been isolated from the culture of Penicillium tropicum and their structures are elucidated as epi-10,23-dihydro-24,25-dehydroaflavinine(1),Penipropone(2),(3 S)-3,5-dimethyl-8-methoxy-3,4-dihydro-1 H-isochromen-6-ol(3),(-)-massoialactone(4)and(3 R,5 R)-3-O-β-D-mannosyl-3,5-dihydroxydecanoic acid(5).Antibacterial activity test reveals that compound(1)shows inhibitory activities against S.aureus USA300 with MIC values of 2.0μg·mL-1.

关 键 词：内生真菌 PENICILLIUM tropicum 抑菌活性 次生代谢产物 

分 类 号：R914.4[医药卫生—药物化学]