钙盐沉淀法从酮酸发酵液中提取α-酮戊二酸  

Extraction ofα-Ketoglutaric Acid from Fermentation Broth by Calcium Precipitation Method

作　　者：彭小雨 曾伟主 周景文[1,2,3] 徐国强 PENG Xiaoyu;ZENG Weizhu;ZHOU Jingwen;XU Guoqiang(School of Biotechnology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2020年第3期65-70,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金重大项目(21390204)。

摘　　要：α-酮戊二酸作为一种重要的有机酸,在食品、化工、医药等领域具有广泛的用途。目前,发酵法是生产α-酮戊二酸最具优势的方法。但在解脂亚洛酵母发酵生产α-酮戊二酸的过程中,丙酮酸作为副产物总是大量积累。这2种酮酸物化性质相似,为下游分离提取过程造成了很大的困难。研究对比3种不同钙盐对酮酸分离的影响,建立了一种有效分离发酵液中α-酮戊二酸的钙盐沉淀方法。采用CaCO3为α-酮戊二酸的最佳沉淀剂,α-酮戊二酸的回收率和纯度分别为82.5%和98.89%。同时,结果表明在偏中性和高温作用下,丙酮酸的稳定性受到影响,丙酮酸分离不适用于钙盐沉淀法。该研究可为酮酸的分离提取过程提供重要的理论参考。α-Ketoglutaric acid is widely used in food,chemical and pharmaceutical industries as an important organic acid.Fermentative production ofα-ketoglutaric acid by Yarrowia lipolytica is currently a competitive method.The accumulation of pyruvic acid is unavoidable during the production ofα-ketoglutaric acid by Y.lipolytica.The two keto acids have similar physical and chemical properties,which brings big challenge for the downstream separation process.In this study,effects of three different calcium salts on the separation of keto acids in the fermentation broth were compared.An effective precipitation method was established for the separation ofα-ketoglutaric acid from fermentation broth.CaCO3 was the optimum precipitant ofα-ketoglutaric acid.The recovery and purity rate ofα-ketoglutaric acid were 82.5%and 98.89%,respectively.Meanwhile,the precipitation method could not be used for the separation of pyruvic acid,because pyruvic acid was unstable under the partial neutrality and high temperature.This study can thus provide an important theoretical reference for the separation and extraction of keto-acid.

关 键 词：钙盐沉淀法 Α-酮戊二酸 丙酮酸 解脂亚洛酵母 有机酸 

分 类 号：Q815[生物学—生物工程]