肠炎沙门氏菌yjeA基因缺失株的构建及其生物学功能测定  

Construction and characterization of yjeA gene deletion strain of Salmonella enteritidis

作　　者：曹莉 孙路 孔梓安 吴竹青 别鹏飞 王真[1] CAO Li;SUN Lu;KONG Zian;WU Zhuqing;BIE Pengfei;WANG Zhen(Beijing University of Agriculture Beijing Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Animal Science and Technology College 102206;China agricultural university Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture,College of Veterinary Medicine,100193)

机构地区：[1]北京农学院动物科学技术学院,兽医学(中兽医)北京市重点实验室,北京102206 [2]中国农业大学动物医学院,北京100193

出　　处：《北京农学院学报》2020年第2期16-20,共5页Journal of Beijing University of Agriculture

基　　金：北京市优秀人才培养资助项目(胞内寄生病原菌抗菌肽耐受与持续性感染相关性研究,2017000026833ZK18);2019年北京市属高校高水平教师队伍建设支持计划——青年拔尖人才培育计划项目(CIT&TCD201904052);北京农学院学位与研究生教育改革与发展项目。

摘　　要：【目的】为了研究yjeA基因的缺失肠炎沙门氏菌的生物学特性和毒力的影响。【方法】以肠炎沙门氏菌为亲本菌株,构建沙门氏菌yjeA基因缺失株,并对其生物学特性进行研究。【结果】利用Red重组系统成功构建沙门氏菌yjeA基因缺失株,进一步生物学特性研究表明,与亲本菌株相比,缺失株SMΔyjeA生长稳定,在细胞内生存能力减弱,小鼠肝脾载菌量降低,并能刺激机体产生特异性抗体。【结论】沙门氏菌突变株SMΔyjeA不仅毒力减毒,并且保留了良好的免疫原性,揭示yjeA基因缺失株有望开发成沙门氏菌减毒疫苗。【Objective】Salmonella Enteritidis is a zoonotic pathogen,which has serious harm to aquaculture and public health;【Methods】In this study,Salmonella enteritidis was used as the parent strain to construct a Salmonella yjeA gene deletion strain,and its biological characteristics were studied;【Results】Compared with the parent strain,the deletion strain SMΔyjeA has a stable growth,a weak ability to survive in cells,a lower amount of bacteria in mice liver and spleen,and can stimulate the production of specific antibodies;【Conclusion】Salmonella mutant SMΔyjeA not only attenuated the virulence,but also retainedgood immunogenicity,revealing that the yjeA gene deletion strain is expected to be developed into a Salmonella attenuated vaccine.

关 键 词：肠炎沙门氏菌 yjeA RED重组系统 毒力 免疫原性 

分 类 号：S855[农业科学—临床兽医学]