银杏叶总黄酮超声辅助提取条件优化及其清除羟自由基能力  

Optimization of Ultrasonic Assisted Extraction for Total Flavonoids from Ginkgo biloba Leaves and Its Scavenging Capacity on Hydroxyl Free Radicials

作　　者：闫旭宇 李玲 YAN Xu-yu;LI Ling(College of Life Science,Yanan University,Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources,Yanan 716000,China;Department of Chemistry and Biological Enguneering,Hunan University of Science and Engineering,Yongzhou 425199,China)

机构地区：[1]延安大学生命科学学院,陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000 [2]湖南科技学院化学与生物工程学院,湖南永州425199

出　　处：《食品工业科技》2020年第9期200-204,224,共6页Science and Technology of Food Industry

基　　金：陕西省区域生物资源保育与利用工程中心开放项目(sxgczx-2019-10);湖南省教育厅科学研究项目(17B108);永州市2016年度指导性科技创新及应用研究项目(永科发[2016]27号)。

摘　　要：本研究以银杏叶为研究对象,采用超声辅助乙醇提取优化了银杏叶总黄酮的提取工艺。在单因素实验基础上,以总黄酮得率为响应值,对影响银杏叶总黄酮得率的4个因素进行Box-Behnken试验设计与优化,分析提取过程中乙醇浓度、料液比、提取温度和提取时间对总黄酮得率的影响,并初步研究了黄酮提取物对羟自由基的清除作用。结果表明,银杏叶总黄酮的最佳提取工艺为:乙醇浓度81%、料液比1∶26 g/mL、提取温度75℃、提取时间51 min,银杏叶总黄酮得率为3.58%。所提取的银杏叶总黄酮对羟自由基的清除效果高于V C,且与浓度正相关。本研究为银杏叶总黄酮的提取与开发利用提供了理论参考。In the present study,Ginkgo biloba leaves were used as the research object,and the extraction process of total flavonoids in Ginkgo biloba leaves was optimized by ultrasound-assisted ethanol extraction.Based on single factor test analysis,the total flavonoids yield was set as the response value.Four factors which affect total flavonoids extraction from Ginkgo biloba leaves were designed and optimized by Box-Behnken experiment,so as to analyze the effect of ethanol concentration,material-to-liquid ratio,extraction temperature and extraction time on the yield during the extraction process.And the scavenging rate of hydroxyl radicals was also studied.The results showed that the optimum extraction technology of total flavonoids from Ginkgo biloba leaves were ethanol concentration 81%,material-to-liquid ratio 1∶26 g/mL,extraction temperature 75℃ and extraction time 51 min.The yield of total flavonoids could be up to 3.58% under the optimal condition.The total flavonoids extracted from Ginkgo biloba leaves had higher scavenging effect on hydroxyl radicals than V C,and its antioxidant capacity had a positive correlation with the concentration.The study could provide theoretical basis for the extraction,utilization and development of total flavonoids from Ginkgo biloba leaves.

关 键 词：银杏叶 黄酮 响应面 羟自由基 清除能力 超声辅助提取 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]