Colonization of Bacillus cereus NJSZ-13,a species with nematicidal activity in Masson pine(Pinus massoniana Lamb.)  

作　　者：Liangliang Li Jiajin Tan Fengmao Chen Dejun Hao 

机构地区：[1]College of Forestry,Nanjing Forestry University,Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing 210037,People’s Republic of China

出　　处：《Journal of Forestry Research》2020年第3期1025-1033,共9页林业研究（英文版）

基　　金：supported by the National Key R&D Program of China[2018YFC1200400];the Nature Science Foundation of the Jiangsu Higher Education Institutions of China[14KJA220002];the Postgraduate Scientific Research and Innovation Program of Jiangsu Province[KYLX16_0859];the National Natural Science Foundation of China[31370643]。

摘　　要：Bacillus cereus NJSZ-13,an endophytic bacterium with nematicidal activity,was isolated from stems of healthy Pinus elliottii Engelm.Colonization of P.massoniana Lamb.by endophytic B.cereus was studied using scanning electron microscopy and confocal laser scanning microscopy.After the plasmid p GFP78 containing the green fluorescent protein(GFP)gene was transformed into the NJSZ-13 strain,the NJSZ-13:gfp showed the same nematicidal activity and growth curve as the wild-type strain,and the plasmid p GFP78 was stably maintained in strain NJSZ-13 for at least 96 h of bacterial cultivation on medium without antibiotics.After inoculation into Masson pine roots,colonization of the NJSZ-13:gfp strain in plant roots and stems was visualized using confocal laser scanning and the strain was enumerated in inoculated roots and stems.These results suggest that NJSZ-13:gfp is an efficient colonizer of Masson pine and can transfer vertically from roots to stems.

关 键 词：Bacillus CEREUS ENDOPHYTES Green fluorescent protein PLASMID stability COLONIZATION 

分 类 号：S763.7[农业科学—森林保护学]