In vitro leishmanicidal activity of antimicrobial peptide KDEL against Leishmania tarentolae  

作　　者：Lili Cao Weina Jiang Songgao Cao Panpan Zhao Juan Liu Hang Dong Yanbing Guo Quan Liu Pengtao Gong 

机构地区：[1]Key Laboratory of Zoonosis Research by Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun 130062,China [2]Department of Parasite,Jilin Academy of Animal Husbandry and Veterinary Medicine,Changchun 130062,China [3]Department of Pathology,Qingdao Municipal Hospital,Qingdao 266071,China [4]Pingdu People's Hospital,Qingdao 266700,China [5]College of Life Sciences and Engineering,Foshan University,Foshan 528000,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第12期1286-1292,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.31672288,31302076,and 31001057);the National Science and Technology Major Project of the Ministry of Science and Technology of China(No.2012ZX09103301-021);the National Key Technology R&D program(No.2015BAI07B02);China Postdoctoral Science Foundation(No.2014 M552636).

摘　　要：Leishmaniasis,caused by the intracellular protozoan parasite Leishmania,remains an important neglected tropical infectious disease.Infection may be lethal if untreated.Currently,the available drugs for the disease are limited by high toxicity and drug resista nee.There is an urgent n eed to develop novel anti-leishmanial strategies.Antimicrobial peptides(AMPs)have been described as the first-line immune defense against pathogenic microbes and are being developed as emerging anti-parasitic therapies.In the present study,we showed the anti-leishmanial activity of the synthetic 4-amino acid peptide lysine,aspartic acid,glutamic acid,and leucine(KDEL),the endoplas?mic reticulum retention sequence,against Leishmania tarentolae promastigote and amastigote.Different concentrations of KDEL peptides were incubated with promastigotes,MTT viability assay,and promastigote assay were carried out.Macrophages infected with GFP-transfected L.tarentolae promastigotes were incubated with KDEL peptides,and the anti-amastigote activity of the KDEL peptides was measured by fluoresce nee microscopy.The damage of L.tarentolae was observed by light microscopy and electron microscopy.The cell apoptosis was analyzed using the Annexin V-FITC/PI apoptosis detection kit and mitochondrial membrane potential assay kit and by flow cytometry.Results showed that L.tarentolae was susceptible to KDEL peptides in a dosedepende nt manner,and KDEL peptides disrupted the surface membrane integrity and caused cell apoptosis.In our study,we found for the first time an AMP KDEL from Pseudomonas aeruginosa and proved its significant therapeutic potential as a novel anti-leishmanial drug.

关 键 词：ANTIMICROBIAL PEPTIDE KDEL LEISHMANIA xarento\ae LEISHMANIASIS 

分 类 号：R73[医药卫生—肿瘤]