超声辅助提取款冬总黄酮工艺及抗氧化活性研究  

Study of Ultrasonic-assisted Extraction Technology and Antioxidant Activity of Total Flavonoids from Tussilago farfara L.

作　　者：陈苏丹[1] 汪亚祺 李秀珍[1] 李学强[1] CHEN Sudan;WANG Yaqi;LI Xiuzhen;LI Xueqiang(Forestry School,Henan University of Science&Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学林学院,河南洛阳471023

出　　处：《河南科技大学学报（自然科学版）》2020年第4期82-87,M0007,共7页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家自然科学基金项目(31601889,61801170);河南省科技攻关基金项目(182102110401);河南省高校创新人才基金项目(20HASTIT022,17HASTIT025);河南科技大学博士科研启动基金项目(13480053)。

摘　　要：采用正交试验优化款冬总黄酮的超声辅助提取工艺,并测定提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O2^-·)的清除作用。研究结果表明:款冬总黄酮的最优提取工艺条件为超声温度50℃,超声时间50 min,乙醇体积分数70%,料液体积比1∶40。在此工艺条件下,款冬总黄酮提取率为8.02%。款冬总黄酮质量浓度为0.06 mg·mL^-1时,对DPPH·、·OH和O2^-·的清除率分别达到81.03%、44.59%和83.34%。款冬总黄酮具有良好的抗氧化能力。Orthogonal test was used to optimize the ultrasonic-assisted extraction technology of total flavonoids from Tussilago farfara,and the clearance effects of the extractive on 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·),hydroxyl free radical(·OH)and superoxide anion free radical(O2^-·)were determination.The research results demonstrates that the optimal extraction technology conditions of total flavonoids from Tussilago farfara are 50℃ of ultrasonic temperature,50 min of ultrasonic time,70% ethanol volume fraction and 1∶40 of material to liquid.Under this technology condition,the extraction rate of total flavonoids from Tussilago farfara is 8.02%.When the mass concentration of total flavonoids from Tussilago farfara is 0.06 mg·mL^-1,the clearance rate to DPPH·,·OH and O2^-·can be reached to 81.03%,44.59% and 83.34%,respectively.The total flavonoids from Tussilago farfara have nice antioxidant ability.

关 键 词：款冬 总黄酮 超声辅助提取 正交试验 抗氧化活性 

分 类 号：S567.23[农业科学—中草药栽培]