Expression,regulation and binding affinity of fatty acid-binding protein 2 in Spodoptera litura  

作　　者：WEN Liang GAO Gui-ping HUANG Zhi-qiang ZHENG Si-chun FENG Qi-li LIU Lin 

机构地区：[1]Institute of Insect Science and Technology,School of Life Sciences,South China Normal University/Guangzhou Key Laboratory of Insect Development Regulation and Applied Research/Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Guangzhou 510631,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第6期1492-1500,共9页农业科学学报（英文版）

基　　金：The study was supported by the National Natural Science Foundation of China(31330071 and 31772519)and the Natural Science Foundation of Guangdong Province,China(2017A030313210).

摘　　要：Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned from Spodoptera litura.RT-PCR and Western blot analysis indicated that RNA and protein levels of S/FABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.The expression of S/FABP2 protein was induced by starvation treatment.In vitro binding assay revealed that the recombinant S/FABP2 had high mffinities of binding Iong-chain fatty acids,such as palmitic acid,arachidonate and oleic acid.The results suggest that S/FABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.This work provides experimental clues for understanding the potential function of S/FABP2 in fatty acid metabolism in S.litura.

关 键 词：SPODOPTERA litura S/FABP2 STARVATION treatment BINDING ASSAY 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]