Characteristics and roles of cytochrome b5 in cytochrome P450-mediated oxidative reactions in Locusta migratoria  

作　　者：LIU Jiao ZHANG Xue-yao WU Hai-hua MA Wen ZHU Wen-ya Kun-Yan ZHU MA En-bo ZHANG Jian-zhen 

机构地区：[1]Institute of Applied Biology,Shanxi University,Taiyuan 030006,P.R.China [2]College of Life Science,Shanxi University,Taiyuan 030006,P.R.China [3]Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,P.R.China [4]Institute of Plant Protection,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,P.R.China [5]Department of Entomology,Kansas State University,Manhattan,KS 66506,USA

出　　处：《Journal of Integrative Agriculture》2020年第6期1512-1521,共10页农业科学学报（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China(31320103921 and 31872010)and the Graduate Outstanding Irinovation Project of Shanxi Province,China(2017 BY011).

摘　　要：Cytochrome b5(Cyt-b5)is a small heme protein and known to be involved in a wide range of biochemical transformations,in eluding cytochrome P450 monooxyge nase(CYP)-mediated metabolism of endoge nous and exogenous compo un ds.Studies on Cyt-b5 are more con centrated in mammals,but are relatively rare in in sects.The characteristics and functi on of Cyt-b5 from Locusta migratoria have not been described yet.We sequeneed the full-length cDNA sequenee of Cyt-b5 from L.migratoria(LmCyt-b5)by reverse transcription-PCR(RT-PCR)based on locust transcriptome database.The phylogenetic analysis showed that LmCyt-b5 was closely related to the Cyt-b5 from Blattodea.LmCyt-b5 was highly expressed in ovary,Malpighian tubules,midgut,gastric caeca,and fat bodies.Silencing of LmCyt-b5 had no effect on the susceptibility of L.migratoria to four different insecticides.Suppression of LmCyt-b5 or silencing of both LmCyt-b5 and LmCPR did not significantly change the total CYP activity toward the substrate 7-ethoxycoumarin(7-EC).However,coexpression of LmCYP6FD1 with LmCPR and LmCyt-b5 together in Sf9 cells by using Bac-to-Bac baculovirus expression system significantly increased the catalytic activity of LmCYP6FD1 toward 7-EC as compared with the coexpression of L.mCYP6FD1 with cytochrome P450 reductase(LmCPR)or LmCyt-b5 separately.These results suggest that LmCyt-b5 plays an important role in the catalytic reaction of LmCYP6FD1 toward 7-EC in our in vitro experiments.Further study is needed to clarify the role of LmCyt-b5 in CYP-mediated catalytic reactions in L.migratoria.

关 键 词：CYTOCHROME b5 CYTOCHROME P450 CYTOCHROME P450 REDUCTASE LOCUSTA MIGRATORIA RNA interference 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治]