超高效液相色谱-质谱联用技术解析黑果枸杞超临界CO2萃取物中黄酮类天然产物结构  

Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

作　　者：束彤 丁丽娜 王茜 贾彩惠 邱亦亦 阮晖[2,3,4] SHU Tong;DING Lina;WANG Qian;JIA Caihui;QIU Yiyi;RUAN Hui(Qinghai Institute for Food Control,Xining 810008,China;College of Biosystems Engineering&Food Science,Zhejiang University,Hangzhou 310058,China;Zhejiang Key Laboratory for Agro-food Processing,Hangzhou 310058,China;Ningbo Research Institute,Zhejiang University,Ningbo 315100,China)

机构地区：[1]青海省食品检验检测院,青海西宁810008 [2]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [3]浙江省农产品加工技术研究重点实验室,浙江杭州310058 [4]浙江大学宁波研究院,浙江宁波315100

出　　处：《食品科学》2020年第10期206-212,共7页Food Science

基　　金：浙江省科技计划项目(2017C26004);青海省科技计划项目(2017-ZJ-Y06);西宁市科技计划项目(2019-Y-09)。

摘　　要：以青海柴达木黑枸杞为研究材料,对其超临界CO2萃取物,采用超高效液相色谱-质谱联用技术和PeakView色谱工作站拟合进行分析,并通过二级质谱解析和紫外光谱及质谱数据比对,阐明其中的黄酮组分及其结构。共鉴定出黑果枸杞果的超临界CO2萃取物中含有13种黄酮类化合物(7种黄酮醇苷元及黄酮醇苷,3种二氢黄酮醇苷元,2种黄酮苷元和1种二氢黄酮苷元),包括芦丁、槲皮素-3-β-葡萄糖醛酸苷、金丝桃苷即槲皮素-3-O-吡喃半乳糖苷、二氢槲皮素、二氢异鼠李素、二氢山柰酚、槲皮素、芹菜素、橙皮素、苜蓿素、山柰酚、异鼠李素和山柰素,其中橙皮素和苜蓿素在黑果枸杞成分研究中未被发现过。The flavonoid components of the supercritical CO2 extract of black Goji berries from Qaidam,Qinghai were analyzed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)employing PeakView workstation,and their structures were determined by analysis of the tandem mass spectra,ultraviolet(UV)spectroscopic analysis and comparison of the mass spectral data with the reference standards.As a result,a total of 13 flavonoids were identified,including 7 flavonol aglycones and flavonol glycosides,3 dihydroflavonol aglycones,2 flavonoid aglycones and 1 dihydrogen,namely rutin,quercetin-3-β-glucuronide,hyperoside,quercetin-3-O-galactopyranoside,dihydroquercetin,dihydroiso rhamnosin,dihydrokaempferol,quercetin,apigenin,hesperetin,alizarin,kaempferol,isorhamnetin,and kaempferol.It is noteworthy that hesperetin and alizarin were found in black Goji for the first time.

关 键 词：黑果枸杞 黄酮 超临界CO2萃取物 超高效液相色谱-质谱联用 

分 类 号：TS201.2[轻工技术与工程—食品科学]