基于DPPH、ABTS法的竹叶花椒抗氧化谱效关系研究  

Research on Spectra-Effect Relationship of Antioxidant Activity of Zanthoxylum armatum DC.Based on DPPH and ABTS Methods

作　　者：赵容 张萌萌 陈茜 王丹[1] 袁星[1] ZHAO Rong;ZHANG Meng-meng;CHEN Xi;WANG Dan;YUAN Xing(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)

机构地区：[1]成都中医药大学,成都611137 [2]四川省中医药科学院,成都610041

出　　处：《中国调味品》2020年第5期7-12,共6页China Condiment

基　　金：四川省中医药管理局花椒专项项目(2018HJZX004);四川省教育厅一般项目(18ZB0190)。

摘　　要：该研究采用HPLC法测定10批次竹叶花椒指纹图谱,并通过DPPH和ABTS法测定其抗氧化活性,以半数清除率(EC50)与共有峰峰面积数据为基础,应用皮尔逊相关分析法和逐步回归分析法得出:10个共有峰中5号和8号峰含量变化与竹叶花椒抗氧化活性呈显著正相关,5号和8号峰代表的未知化合物可能是其抗氧化活性的物质基础。研究结果表明,竹叶花椒具有较高的体外抗氧化活性,具备天然抗氧化剂开发价值,物质基础的挖掘为其食品的精深加工发展提供了科学依据。In this study,HPLC method is used to determine the fingerprint spectra of 10 batches of Zanthoxylum armatum DC.,and the antioxidant activity is determined by DPPH and ABTS.Based on the half clearance rates(EC50)and the data of common peaks’peak areas,Pearson correlation analysis and stepwise regression analysis are used to obtain that the content changes of No.5 and No.8 peaks among the 10 common peaks are significantly positively correlated with the antioxidant activity of Zanthoxylum armatum DC..The unknown compounds represented by No.5 and No.8 peaks may be the material basis for their antioxidant activities.The results show that Zanthoxylum armatum DC.has higher antioxidant activity in vitro and has the development value of natural antioxidants.The excavation of material basis has provided scientific basis for the development of its food intensive processing.

关 键 词：竹叶花椒 指纹图谱 抗氧化活性 皮尔逊相关分析 逐步回归分析 

分 类 号：TS264.3[轻工技术与工程—发酵工程]