6种乳酸菌发酵液对皮肤抗氧化及美白功能的评价  

Evaluation of six lactobacillus fermentation broth on skin antioxidant and whitening function

作　　者：邓逸琳 王斌[1,2,3,4] 曹辉 DENG Yi-lin;WANG bin;CAO Hui(College of Biological Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China;National Research and Development Center for Energy Biorefining Engineering,Beijing 100029,China;Beijing Key Laboratory of Biological Processing,Beijing University of Chemical Technology,Beijing 100029,China;Qinhuangdao Bohai Biological Research Institute of Beijing University of Chemical Technology,Qinhuangdao,Hebei 066000,China)

机构地区：[1]北京化工大学生命科学与技术学院,北京100029 [2]国家能源生物炼制工程研发中心,北京100029 [3]北京化工大学北京市生物加工过程重点实验室,北京100029 [4]北京化工大学秦皇岛环渤海生物产业研究院,河北秦皇岛066000

出　　处：《日用化学工业》2020年第5期325-330,共6页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金项目(21865026);新疆生产建设兵团项目(2018BC005);河北省重点研发计划项目(18273401D);河北省创新能力提升计划项目(18952814D)。

摘　　要：考察并比较了6种乳酸菌发酵上清液中多肽和胞外多糖含量,发现Lactobacillus plantarum N2发酵上清液中多肽含量最高可达1.7 g/L,Lactobacillus plantarum N4发酵上清液中胞外多糖含量最高可达5.0 g/L。通过ABTS+自由基清除能力、ROS自由基清除能力和对SOD酶活性、CAT酶活性的影响来评价乳酸菌抗氧化能力,通过对酪氨酸酶的抑制率和对细胞内黑色素含量的影响来评价种乳酸菌的美白能力。实验结果表明,乳酸菌发酵上清液具有一定的抗氧化能力和美白能力,其中N2和N4的自由基清除活性皆高于Vc组,N2和N4对酪氨酸酶的抑制率分别可达到71%和68%。N2和N4具有较好的抗氧化和美白能力。The content of polypeptide and exopolysaccharide in the fermentation supernatant of six kinds of lactic acid bacteria was investigated and compared.It was found that the content of polypeptide in the supernatant of Lactobacillus plantarum N2 was up to 1.7 g/L,and the content of exopolysaccharide in the supernatant of Lactobacillus plantarum N4 was up to 5.0 g/L.The antioxidant capacity of six kinds of lactic acid bacteria was evaluated by ABTS+free radical scavenging capacity,ROS free radical scavenging capacity and the influence on SOD enzyme activity and CAT enzyme activity.Then,the whitening ability of six kinds of Lactobacillus was evaluated by the inhibition rate of tyrosinase and the effect on the content of melanin in cells.The results show that FMS has certain antioxidant and whitening ability,in which the free radical scavenging activities of N2 and N4 were higher than those of Vc group.The inhibition rates of N2 and N4 to tyrosinase were 71%and 68%,respectively.N2 and N4 have good antioxidant and whitening ability.

关 键 词：乳酸菌 多肽 多糖 抗氧化 美白 

分 类 号：TQ658[化学工程—精细化工]