Development and Evaluation of a Universal and Supersensitive NS1-Based Luciferase Immunosorbent Assay to Detect Zika Virus-Specific IgG  

作　　者：Tianyu Wang Ying Zhan De Wu Zhihai Chen Wei Wu Yao Deng Wenling Wang Wenjie Tan Shixing Tang 

机构地区：[1]Guangdong Provincial Key Laboratory of Tropical Disease Research,Department of Epidemiology,School of Public Health,Southern Medical University,Guangzhou 510515,China [2]NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,China CDC,Beijing 102206,China [3]Key Laboratory for Repository and Application of Pathogenic Microbiology,Research Center for Pathogens Detection Technology of Emerging Infectious Diseases,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430,China [4]The National Clinical Key Department of Infectious Disease,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China

出　　处：《Virologica Sinica》2020年第1期93-102,共10页中国病毒学（英文版）

基　　金：the National Key Research and Development Program of China(2016YFD0500301);the National Major Project for Control and Prevention of Infectious Disease in China(2018ZX10101002 and 2017YFC1200503);the Bureau of Science and Information Technology of Guangzhou Municipality,China(201604020011,201704020219);National Key R&D Program of China(2018ZX10732401).

摘　　要：Zika virus(ZIKV) causes rash, moderate fever, conjunctivitis, and arthralgia, and has serious connection with neurological complications;therefore, it is a major threat to public health. A rapid and supersensitive method for detecting anti-ZIKV antibodies in humans and animals is thus urgently required. Here, we report an NS1-based luciferase immunosorbent assay(LISA), developed to detect ZIKV-specific IgG. Fusion proteins including a reporter Nano-luciferase(NLuc) and various fragments of ZIKV NS1 protein were expressed in 293 T cells. LISA was performed using the above cell lysates containing the expressed fusion proteins. Sample panels of humans and animals infected with ZIKV were examined for sensitivity of LISA, relative to those of ZIKV RT-PCR, commercial NS1-based ELISA, and micro-neutralization(MN) assays.Specificity and potential cross-reactivity were also evaluated using various convalescent serum samples derived from patients infected with dengue virus(DENV), Japanese encephalitis virus(JEV), and hepatitis C virus(HCV). Results indicated the optimal antigenic domain for anti-ZIKV IgG detection was located within 172–352 amino acids(aa) of ZIKV NS1 protein. NS1-based LISA performs better than commercial ELISA in anti-ZIKV Ig G detection. LISA was shown to be at least fourfold more sensitive than commercial ELISA, and could detect anti-ZIKV Ig G in various animal hosts without the need of species-specific labeled antibody. This novel assay is potentially useful for the rapid and sensitive detection of anti-ZIKV IgG in human and animal samples.

关 键 词：Zika virus(ZIKV) NS1 LUCIFERASE IMMUNOSORBENT ASSAY IgG Detection 

分 类 号：R511[医药卫生—内科学] R446.6[医药卫生—临床医学]