Experimental study on the relationship between traumatic stress and tumor growth,proliferation,and metastasis  

作　　者：Weigang Cao Baoan Qiu 

机构地区：[1]Department of Breast and Thyroid Surgery,Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264100,China [2]Department of Hepatobiliary Surgery,The Sixth Medical Center of PLA General Hospital,Beijing 100048,China

出　　处：《Oncology and Translational Medicine》2020年第2期52-56,共5页肿瘤学与转化医学（英文版）

基　　金：a grant from yantai City Key R&D Projects(2019YD063);Science and Technology Project of Binzhou Medical University(BY2018KJ31).

摘　　要：Objective This study aimed to investigate the relationship between traumatic stress and tumor growth,proliferation,and metastasis.Methods A scalding method was used as an injurious factor to induce traumatic stress in Wistar rats.The rats were randomly divided into three groups—the control group,mild-scald group,and severe-scald group,with 14 rats in each group.Wistar rats were used to subculture the Walker-256 cell line for the generation of tumor ascites.Tumor cells from the ascites were cultured and used to establish a rat subcutaneous xenograft model.After 7 days,the mild-burn group and the severe-burn group were subjected burns to 10%and 15%of their backs,respectively.Blood was taken from the tail vein of rats at different times to detect changes in blood cortisol,IL-1β,and TNF-αlevels.Pathological specimens were collected 14 days later,and immunohistochemistry was performed to examine vascular endothelial growth factor(VEGF),proliferating cell nuclear antigen(PCNA),E-cadherin,and vimentin.Results Cortisol,IL-1βand TNF-αlevels were significantly higher in the scalding groups than in the control group.Tumor examination was performed after 14 days.The changes in tumor size showed that the tumor volume in the control group(0.593±0.195 cm3)and the mild-scald group(0.782±0.344 cm3)were not significantly different.However,the tumor volume was significantly larger in the severeburn group(1.806±0.838 cm3)than in the control and the mild-burn groups(P<0.05).Tumor tissue immunohistochemistry showed that the percentage of cells expressing PCNA in the control group,mildscald group,and severe-scald group was 57.1%,71.4%and 85.7%,respectively,and the differences among the groups were statistically significant.The number of VEGF-positive cells in the mild-and severescald groups was significantly higher than those of the control group(P<0.05).The number of E-cadherinpositive cells in the tumor tissues was significantly lower in the severe-scald group than that in the control and mild-scald groups.Vimentin showed th

关 键 词：stress response Wistar rats TUMOR SCALDING 

分 类 号：R730.2[医药卫生—肿瘤]