添加Fe^2+对克雷伯氏菌发酵产氢特性的影响  

Effect of ferrous iron supplementation on characteristics of fermentative hydrogen production by Klebsiella sp.

作　　者：丁鹏飞 李艳宾 蒋卉[2] 张琴 DING Peng-fei;LI Yan-bin;JIANG Hui;ZHANG Qin(College of Biological and Chemical Engineering,Anhui Polytechnic University,Wuhu 241000,China;College of Life Science,Tarim University,Alaer 843300,China)

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《应用化工》2020年第4期832-835,共4页Applied Chemical Industry

基　　金：国家自然科学基金项目(21406150);安徽省重点研究与开发项目(201904a07020003);安徽工程大学引进人才科研启动基金(2018YQQ029)。

摘　　要：在克雷伯氏菌发酵棉秆水解糖液产氢体系中添加Fe 2+,探究不同Fe 2+添加浓度对菌株产氢及其动力学、还原糖利用、生长OD值及其糖利用耦合的产氢和菌体生长效率的影响。结果表明,Fe 2+添加浓度为20 mg/L处理的累积产氢量最高,达2200 mL/L以上,较之0 mg/L处理的提高约20%,Gompertz模型拟合的参数P、R m值最高,分别可达2550.63 mL/L、25.08 mL/(L·h)。该添加浓度下,菌株获得的葡萄糖、木糖利用率和ΔOD 600值最高,较之0 mg/L处理的分别提高12%,4.3%和6.5%,基于还原糖利用的产氢效率Y(H 2/S)亦提高7.9%。Ferrous iron was supplemented in the fermentative hydrogen production system of cotton stalk hydrolysate by Klebsiella sp.,the effects of different addition concentrations of Fe 2+on hydrogen production and dynamics,reducing sugar utilization,bacterial growth,as well as the hydrogen production and bacterial growth efficiency based on sugar utilization were explored.The results showed that in the treatment with addition of 20 mg/L Fe 2+,the highest cumulative hydrogen production was achieved over 2200 mL/L,which was about 20%higher than the treatment with 0 mg/L Fe 2+addition,and the Gompertz model fitted parameters P and R m value were highest,which were reached 2550.63 mL/L and 25.08 mL/(L·h)respectively.Moreover,the optimum glucose,xylose utilization andΔOD 600 value were also obtained at this concentration,which were enhanced of 12%,4.3%and 6.5%separately comparing with the treatment with 0 mg/L Fe 2+addition,even the hydrogen production efficiency based on sugar utilization Y(H 2/S)was also increased 7.9%under this addition concentration.

关 键 词：克雷伯氏菌 Fe 2+添加 棉秆水解糖液 产氢 发酵 

分 类 号：TQ929[轻工技术与工程—发酵工程] TQ033