一株产几丁质脱乙酰酶海洋细菌的Rhodococcus hoagii筛选、鉴定及酶学性质  

Screen,identification and enzymatic properties of a marine bacteria strain producing chitin deacetylase

作　　者：王敏卜 柴金龙 武波飞 苏永成 王淑军[2] 焦豫良 杨杰[3] 房耀维 刘姝 WANG Min-bo;CHAI Jin-long;WU Bo-fei;SU Yong-cheng;WANG Shu-jun;JIAO Yu-liang;YANG Jie;FANG Yao-wei;LIU Shu(College of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang 222005;Jiangsu Marine Resources Development Research Institute,Lianyungang 222000;School of Marine Resources and Environment,Jiangsu Ocean University,Lianyungang 222005)

机构地区：[1]江苏海洋大学海洋生命与水产学院,连云港222005 [2]江苏省海洋资源开发研究院,连云港222005 [3]江苏海洋大学海洋资源与环境学院,连云港222005

出　　处：《中国食品添加剂》2020年第5期63-68,共6页China Food Additives

基　　金：国家自然科学基金项目(31772016);江苏省海洋科技创新专项(HY2018-10);江苏省“六大人才高峰”第十二批高层次人才项目(SWYY-195);江苏省“333高层次人才培养工程”;连云港市“521高层次人才培养工程”。

摘　　要：从连云港高公岛海域采集的海泥中使用平板变色圈法初筛和摇瓶发酵复筛后,获得一株产几丁质脱乙酰酶的菌株MCDAⅡ-2。综合形态学特征以及16SrDNA序列分析最终将菌株MCDAⅡ-2鉴定为Rhodococcus hoagii。进而研究其酶学性质,得该几丁质脱乙酰酶最适催化温度为35℃,最适pH为8.0。Na^+、Mg^2+、Li+、Sr^2+对酶促反应起到促进作用,Ca^2+、Ba^2+、Co^2+、Cd^2+、Fe^2+及EDTA对酶促反应起到了抑制作用,而K^+对酶促反应影响较小。酶在25℃~35℃时较稳定,在中性和弱碱性条件下稳定性较好。本次研究的结果将为几丁质脱乙酰酶的工业化应用奠定基础。MCDAⅡ-2,a chitin-deacetylase-producing strain,was screened from sea mud collected from Gaogong Island in Lianyungang by flat-plate color circle method and re-screened by flask shaking fermentation.The strain MCDAⅡ-2 was identified as Rhodococcus hoagii based on morphological characteristics and 16 S rDNA sequence analysis.Optimum temperature 35℃and pH of chitin deacetylase was 8.0 respectively.Metal ions such as Na^+,Mg^2+,Li+and Sr^2+were found to improve the enzyme activity compared to a control.The experiment also showed that Ca^2+,Ba^2+,Co^2+,Cd^2+,Fe^2+can enhance the enzyme activity,while EDTA and K^+had little effect.The enzyme performed well in a temperature range of 25℃~35℃,with good stability under neutral and weak base conditions.The results of this study will lay a foundation for the industrial application of the enzyme.

关 键 词：几丁质脱乙酰酶 菌株筛选 鉴定 酶学性质 

分 类 号：Q939.97[生物学—微生物学] TS202.3[轻工技术与工程—食品科学]