蒜头果蛋白的纯化鉴定及分析  

Purification,identification and analysis of malanin from Malania oleifera

作　　者：杨敏[1,2] YANG Min(Institute of Flavour and Fragrance,Yunnan Agriculture University,Kunming 650201,China;Key Laboratory of Medicinal Chemistry for Natural Resource,Yunnan University,Kunming 650091,China)

机构地区：[1]云南农业大学香料研究所,昆明650201 [2]云南大学教育部自然资源药物化学重点实验室,昆明650091

出　　处：《中国油脂》2020年第4期24-27,31,共5页China Oils and Fats

摘　　要：采用粉碎、磷酸盐缓冲溶液浸提、硫酸铵沉淀、疏水色谱层析等分离手段,从蒜头果种仁中分离纯化蒜头果蛋白,并对其相对分子质量、氨基酸组成、等电点、中性糖含量、自由巯基含量、细胞活性等进行分析。结果表明:蒜头果蛋白是一种糖蛋白,含有18种氨基酸,等电点为4.6,相对分子质量为61875 Da,由A、B 2条链通过二硫键连接而成,蒜头果蛋白对Hela和Vero细胞具有强烈的细胞毒性。蒜头果蛋白作为一种新的蛋白质,有较好的药用价值。Malanin was isolated from Malania oleifera seed kernel by homogenization,extraction with phosphate buffered solution,precipitation by ammonium sulfate and hydrophobic chromatography.The relative molecular weight,amino acid composition,isoelectric point,neutral sugar content,free sulfhydryl content and cell activity were analyzed.The results showed that the malanin was a glycoprotein containing 18 kinds of amino acids,with relative molecular weight of 61875 Da and isoelectric point of 4.6,and it was linked with two bands of A-chain and B-chain by one or more disulfide bonds.The cytotoxicities of malanin to Hela and Vero cells were very strong.The malanin was a novel protein with good medicinal value.

关 键 词：蒜头果 蒜头果蛋白 分离纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学] TQ028[轻工技术与工程—食品科学与工程]