基于β-环糊精与偶氮苯衍生物的基因传递体系构筑  

Construction of Photo-responsive Gene Delivery System Based on β-Cyclodextrin and Azobenzene Derivatives

作　　者：唐强 张弘 周宝晗 徐保明 TANG Qiang;ZHANG Hong;ZHOU Bao-han;XU Bao-ming(School of materials and chemical engineering,Hubei university of technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学材料与化学工程学院,武汉430068

出　　处：《高分子通报》2020年第3期40-46,共7页Polymer Bulletin

基　　金：湖北省教育厅科学技术研究项目(B2018041)。

摘　　要：通过β-环糊精修饰聚乙烯亚胺(HPEI-CDs)与偶氮苯修饰聚乙二醇(Azo-PEG)的主客体作用,制备了HPEI-CDs/Azo-PEG/DNA自组装体,并对其生理盐溶液稳定性、粒径形貌及光控特性进行了研究。结果表明,对比HPEI-CDs/DNA自组装体,PEG的引入显著提高了其生理盐稳定性,借助PEG壳层的电荷屏蔽和CD氢键的协同作用,自组装体表面电位仅为+3mV,粒径分布均匀,呈现球形结构。在365nm波长光照下,HPEI-CDs/Azo-PEG/DNA的表面电位迅速增加,随着光照时间的延长,其ζ电位最终增大到与HPEI-CDs/DNA相近水平,这表明偶氮苯(Azo)空间结构发生转变,PEG层成功从组装体脱离。Based on the host-guest interaction,HPEI-CDs/Azo-PEG/DNA polyplexes was prepared byβ-cyclodextrin modified with polyethyleneimine(HPEI-CDs)and azobenzene-conjugated with polyethylene glycol(Azo-PEG).The stability of its physiological salt condition,size,morphology and photo response were studied.The results showed that compared with HPEI-CDs/DNA polyplexes,the introduction of PEG significantly improved the stability under physiological salt condition.With the synergistic effect of PEG shell charge screening and CD hydrogen bonding,the surface potential of polyplexes was only+3mV,uniform and spherical microparticles were achieved.The surface potential of HPEI-CDs/Azo-PEG/DNA increased rapidly under 365nm wavelength illumination.With the extension of illumination time,ζpotential eventually increased to a level close to that of HPEI-CDs/DNA,it indicated that azobenzene(Azo)was transformed from trans to cis-structure,light irradiation made Azo-CD inclusion complexes disassemble and lead to PEG shell detachment.

关 键 词：光响应 自组装 Β-环糊精 基因传递 

分 类 号：O641.3[理学—物理化学]