基于转录组测序数据对Weissella confusa XU1中低聚木糖代谢系统的分析  

Transcriptome analysis of xylo-oligosaccharides utilization systems in Weissella confusa XU1

作　　者：戚家明 张东旭[1,2,3] 王少丽 黄立斌 夏丽丽 董汪洋[1] 郑强[1] 刘青娥[1] 肖建中[1] 徐志文[2,3] Jiaming Qi;Dongxu Zhang;Shaoli Wang;Libin Huang;Lili Xia;Wangyang Dong;Qiang Zheng;Qing’e Liu;Jianzhong Xiao;Zhiwen Xu(College of Ecology,Lishui University,Lishui 323000,Zhejiang Province,China;Leading Bio-Agricultural Co.,Ltd.,Qinhuangdao 066000,Hebei Province,China;Engineering&Technology Research Center of Agricultural Biotechnology of Hebei Province,Qinhuangdao 066000,Hebei Province,China)

机构地区：[1]丽水学院生态学院,浙江丽水323000 [2]领先生物农业股份有限公司,河北秦皇岛066000 [3]河北省农业生物技术工程技术研究中心,河北秦皇岛066000

出　　处：《微生物学报》2020年第5期912-923,共12页Acta Microbiologica Sinica

基　　金：浙江省自然科学基金(LY15C200012);丽水市高层次人才项目(2016RC01);浙江省重点研发计划(2018C02031)。

摘　　要：【目的】近年来由于在发酵方面的良好特性,低聚木糖的益生作用越来越得到公众的关注。研究发现相比于葡萄糖和木糖WeissellaconfusaXU1在以低聚木糖为唯一碳源时生长情况最好。本文将对WeissellaconfusaXU1中低聚木糖的代谢机制进行研究。【方法】本研究分别以葡萄糖、木糖和低聚木糖作为唯一碳源对Weissella confusa XU1进行转录组测序并进行比较分析。【结果】通过转录组分析发现以低聚木糖为唯一碳源的处理中部分编码MFS转运蛋白和糖基水解酶的基因转录水平显著上升,WeissellaconfusaXU1中的糖酵解过程和磷酸戊糖途径也得到显著增强。【结论】本研究根据转录组数据分析得出Weissella confusa XU1中的低聚木糖代谢机制。本研究首次在革兰氏阳性菌中发现MFS转运蛋白参与到低聚木糖转运的过程,为提高微生物对木聚糖利用效率进行分子改造提供了改造方向,该机制为低聚木糖代谢的研究和Weissella的工业化应用提供了新的思路。[Objective]The prebiotic effect of xylo-oligosaccharides(XOS)has obtained much attention in recent years due to their fermentation properties.In this study,a kimchi-derived strain,Weissella confusa XU1 grew better on XOS than on glucose and xylose.The mechanism of XOS utilization by W.confusa XU1 was further explored.[Methods]Differential transcriptomes of W.confusa XU1,induced by XOS,glucose and xylose,were analyzed to identify the genetic loci involved in the uptake and catabolism of XOS.[Results]Transcriptome analysis reveals that several major facilitator superfamily(MFS)transporters and glycoside hydrolases were involved in the uptake and hydrolysis of XOS.In addition,glycolysis pathway and pentose phosphate pathway in W.confusa XU1 were enhanced when XOS were present,indicating that XOS were utilized more efficiently compared with other carbon sources.[Conclusion]This study reveals a proposed XOS utilization mechanism in W.confusa XU1.

关 键 词：MFS转运蛋白 益生菌 转录组 Weissella confusa 低聚木糖 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]