pH敏感性可注射型醛基瓜尔豆胶-托普霉素自愈抗菌水凝胶及其性质  

Injectable pH-sensitive Antibacterial Hydrogel Based on Aldehyde-Guar and Tobramycin and Its Properties

作　　者：程淼 王怀生[1] 程霜[2] Cheng Miao;Wang Huaisheng;Cheng Shuang(College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng 252000,China;College of Agriculture,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城大学化学化工学院,山东聊城252000 [2]聊城大学农学院,山东聊城252000

出　　处：《南京师大学报（自然科学版）》2020年第2期34-42,共9页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21375055).

摘　　要：微生物感染是当今严重危害人类健康、威胁人类安全的公共卫生问题之一,有效抑制微生物感染特别是抑制其耐药性是当今研究热点,抗生素水凝胶作为一种新型生物响应医学材料,能实现缓释靶位给药,达到清除微生物的目的.本研究以氧化瓜尔豆胶作为生物兼容性好的可降解天然多糖为骨架,通过席夫碱反应(可逆亚胺键)接枝托普霉素制备了一种可自愈、原位注射成型的酸敏性抗菌水凝胶,并研究了它们的流变学、自愈能力、可注射性、pH响应性、生物相容性和体外抗菌性能.结果表明,凝胶能负载相当量的托普霉素,凝胶具有优异的自愈性能、良好的切稀和可注射性,呈现酸敏给药模式.扫描电镜显示所得水凝胶成多孔网络结构,适合药物、细胞或生物大分子的运载,适于微创抗感染治疗手术的实施,具有潜在的应用价值.Microbial infection is one of the underlying causes of death in the world,which imposes the threat to the public health. To fight against infection and overcome the resistance of microbial has drawn great research interest in materials science and engineering. Polymer hydrogels based on antibiotics have been highly pursued due to the excellent antimicrobial ability,biocompatibility,biodegradability and negligible toxicity. The stimuli-responsive characteristic,bio-inspired functions and rheological properties of the hydrogel which was facilely prepared simply by fabricating aldehyde-guar gum and tobramycin in situ deposition,with its antibacterial ability against E.coli and cytocompatiblility were extensively evaluated in this paper. The results reveal that the smart injectable self-healing hydrogel prepared through the dynamic Schiff base possesses satisfactory rheological properties and sufficient drug loading capability,which presents a self-healing acid sensitive tobramycin sustained release profile after inject in situ and can be used as a practical platform for drug-delivery with distinct applications of antimicrobial. The composite hydrogel is exceptionally propitious to accelerate treatment potential of infection for its porous microstructure,it also is the appropriate biocarrier for cell,drug and macromolecules delivery in the biomedical field.

关 键 词：醛基瓜尔豆胶 托普霉素 水凝胶 流变学 可注射 自愈 PH敏感性 抗菌活力 

分 类 号：TS201.7[轻工技术与工程—食品科学]