Graphene quantum dots rescue protein dysregulation of pancreatic β-cells exposed to human islet amyloid polypeptide  

作　　者：Ava Faridi Yunxiang Sun Monika Mortimer Ritchlynn R.Aranha Aparna Nandakumar Yuhuan Li Ibrahim Javed Aleksandr Kakinen Qingqing Fan Anthony W.Purcell Thomas P.Davis Feng Ding Pouya Faridi Pu Chun Ke 

机构地区：[1]ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,Monash Institute of Pharmaceutical Sciences,Monash University,381 Royal Parade,Parkville,VIC,3052,Australia [2]Department of Physics,Ningbo University,Ningbo,315211,China [3]Department of Physics and Astronomy,Clemson University,Clemson,SC,29634,USA [4]Institute of Environmental and Health Sciences,College of Quality and Safety Engineering,China Jiliang University,Hangzhou,310018,China [5]Infection and Immunity Program&Department of Biochemistry and Molecular Biology,Biomedicine Discovery Institute,Monash University,Clayton,Victoria,3800,Australia [6]Australian Institute for Bioengineering and Nanotechnology,The University of Queensland,Brisbane,Qld,4072,Australia

出　　处：《Nano Research》2019年第11期2827-2834,共8页纳米研究（英文版）

基　　金：This work was conceived by PCK,and was by supported by ARC Project No.CE140100036(Davis),NSF CAREER CBET-1553945(Ding),NIH MIRA R35GM119691(Ding),AFTAM Research Collaboration Award(Davis and Ke),the National Natural Science Foundation of China(No.11904189)(Sun);the Juvenile Diabetes Research Foundation(Purcell and Faridi).A.W.P.is supported by a Principal Research Fellowship from the Australian NHMRC.TEM imaging was performed at Bio21 Advanced Microscopy Facility,University of Melbourne.HIM imaging was performed at the MCFP platform,University of Melbourne by Dr.Anders Barlow.

摘　　要：The amyloid aggregation of peptides and proteins is a hallmark of neurological disorders and type 2 diabetes.Human islet amyloid polypeptide(IAPP),co-secreted with insulin by pancreaticβ-cells,plays dual roles in both glycemic control and the pathology of type 2 diabetes.While IAPP can activate the NLRP3 inflammasome and modulate cellular autophagy,apoptosis and extracellular matrix metabolism,no data is available concerning intracellular protein expression upon exposure to the polypeptide.More surprisingly,how intracellular protein expression is modulated by nanoparticle inhibitors of protein aggregation remains entirely unknown.In this study,we first examined the changing proteomes ofβTC6,a pancreaticβ-cell line,upon exposure to monomeric,oligomeric and fibrillar IAPP,and detailed cellular protein expression rescued by graphene quantum dots(GQDs),an IAPP inhibitor.We found that 29 proteins were significantly dysregulated by the IAPP species,while majority of these proteins were nucleotide-binding proteins.Collectively,our liquid chromatography tandem-mass spectrometry,fluorescence quenching,helium ion microscopy,cytotoxicity and discreet molecular dynamics simulations data revealed a remarkable capacity of GQDs in regulating aberrant protein expression through H-bonding and hydrophobic interactions,pointing to nanomedicine as a new frontier against human amyloid diseases.

关 键 词：islet amyloid polypeptide(IAPP) OLIGOMER amyloid protein expression graphene quantum dot 

分 类 号：R73[医药卫生—肿瘤]