水相酶解法提取柚籽油的研究  

Study on Pomelo Seed 0il Extraction by Aqueous Enzymatic Method

作　　者：武玲 胡爱军[1] 陈文[1] 郑捷[1] WU Ling;*HU Aijun;CHEN Wen;ZHENG Jie(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Musim Mas(Tianjin)Oils&Fats Co.,Ltd.,Tianjin 300452,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]春金(天津)油脂有限公司,天津300452

出　　处：《农产品加工》2020年第10期30-33,共4页Farm Products Processing

摘　　要：柚籽是柚加工的副产物,其开发利用具有重要意义.以柚籽为原料,采用水相酶解法提取柚籽油,研究了酶种类、料液比、酶解温度、酶解时间及酶解pH值对柚籽油提取率的影响.以提取率为考查指标,采用单因素试验和正交试验对提取条件进行优化,得到最佳工艺条件为采用碱性蛋白酶和复合果胶酶混合酶,料液比1∶5(g∶mL),酶解温度50℃,酶解时间4h,酶解pH值5.0.在该工艺条件下,柚籽油的提取率为76.08%.研究结果为柚籽的深加工提供了有价值的技术支撑.Pomelo seed is the byproduct from pomelo processing,its development and utilization has important significance.In this work,pomelo seed was used as raw material to extract the oil by using aqueous enzymatic method.The effects of factors on the oil extraction yield were investigated,including enzyme kinds,solid-liquid ratio,enzymatic temperature,enzymatic time and enzymatic pH.With the extraction yield as the index,both single factor experiment and orthogonal experiment were used to optimize the extraction conditions,the results indicated that the optimal enzymatic conditions for the oil extraction were as follow:combination of alkaline protease and compound pectinase was adequate,solid-liquid ratio was 1∶5(g∶mL),enzymatic temperature 50℃,reaction time 4 h,and pH value 5.0.Under these conditions,the extraction yield of pomelo seed oil was 76.08%.These research results could provide a valuable technical support for the deeply processing of pomelo seed.

关 键 词：水相酶解法 柚籽油 提取 碱性蛋白酶 复合果胶酶 

分 类 号：TQ936[化学工程]