引起肝脓肿的肺炎克雷伯菌毒力基因检测及同源性分析  

Virulence gene detection and homology analysis of Klebsiella pneumoniae inducing liver abscess

作　　者：席健峰 徐义 郭宇航[3] 李慧玲[3] 王勇[3] XI Jianfeng;XU Yi;GUO Yuhang(Jiamusi University, Jiamusi, Heilongjiang 154007, China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]云台山风景名胜区云台街道卫生院,江苏连云港222000 [3]佳木斯大学附属第一医院检验科,黑龙江佳木斯154007

出　　处：《临床肝胆病杂志》2020年第5期1072-1076,共5页Journal of Clinical Hepatology

基　　金：黑龙江省卫生计生委科研课题(2018-291);佳木斯大学校长创新创业基金项目(XZFY 2018-43);佳木斯大学校级课题(S2014-0017)。

摘　　要：目的探讨院内肝脓肿肺炎克雷伯菌荚膜血清型及毒力基因携带情况,且对这些菌株进行同源性分析。方法收集佳木斯大学附属第一医院2018年10月-2019年10月从肝脓肿患者分离出的肺炎克雷伯菌非重复菌株26株,且这些菌株经Vitek-2 Compact全自动微生物分析仪鉴定是肺炎克雷伯菌,对这些菌株进行拉丝试验,使用PCR方法检测这些菌株的主要荚膜血清型及相关毒力基因;并通过多位点序列分型技术分析菌株同源性。计数资料组间比较采用Fisher’s确切检验。结果26株肝脓肿肺炎克雷伯菌拉丝试验阳性率达100%;检测出K1型(17株)、K2型(5株)、K5型(1株)、K57型(1株)、未知血清型2株。毒力基因rmpA、aero、ureA阳性率达100%(26/26);uge、mrkD阳性率为96.2%(25/26);fimH阳性率为80.8%(21/26);iucB阳性率为73.1%(19/26);wcaG、magA、kfu阳性率为65.4%(17/26);allS阳性率为61.5%(16/26);kpn阳性率为30.8%(8/26);iroNB阳性率为7.7%(2/26);未检出cf29a基因;wcaG、magA、allS只在K1血清型中检出;uge未在K57血清型菌中检出。多位点序列分型发现ST23型(17株)、ST86型(3株)、ST65型(2株)、ST1934型(2株)、ST485型(1株)、ST592型(1株)。结论本实验菌株以K1、K2血清型为主,ST23型为本院主要感染序列型。Objective To investigate the serotype and virulence genes of Klebsiella pneumoniae capsule for in-hospital liver abscess,as well as the homology of these strains.Methods A total of 26 non-repetitive strains of Klebsiella pneumoniae isolated from the patients with liver abscess in The First Affiliated Hospital of Jiamusi University from October 2018 to October 2019 were collected.These strains were identified to be Klebsiella pneumonia by Vitek-2 Compact automatic microbiological analyzer.The string test was performed for these strains;PCR was used to determine the major capsular serotypes and related virulence genes;multi-locus sequence typing was used to analyze the homology of the strains.The Fisher’s exact test was used for comparison of categorical data between groups.Results The positive rate of all 26 strains of Klebsiella pneumoniae was 100%in the string test,with 17 strains of K1 type,5 strains of K2 type,1 strain of K5 type,1 strain of K57 type,and 2 strains with unknown serotype.The virulence genes rmpA,aero,and ureA had a positive rate of 100%(26/26);uge and mrkD had a positive rate of 96.2%(25/26);fimH had a positive rate of 80.8%(21/26);iucB had a positive rate of 73.1%(19/26);wcaG,magA,and kfu had a positive rate of 65.4%(17/26);allS had a positive rate of 61.5%(16/26);kpn had a positive rate of 30.8%(8/26);iroNB had a positive rate of 7.7%(2/26).The cf29a gene was not detected;wcaG,magA,and allS were only detected in K1 serotype;uge was not detected in K57 serotype.Multi-locus sequence typing found 17 trains with ST23 type,3 strains with ST86 type,2 strains with ST65 type,2 strains with ST1934 type,1 strain with ST485 type,and 1 strain with ST592 type.Conclusion K1 and K2 serotypes are the main serotypes in the strains in this experiment,and ST23 type is the main sequence type for infection in our hospital.

关 键 词：肝脓肿 肺炎克雷伯菌 基因检测 序列同源性 

分 类 号：R575.4[医药卫生—消化系统]