单分子纳米孔测序技术及其应用研究进展  

Research progress and application of nanopore sequencing technology

作　　者：曹影 李伟[2] 褚鑫[3] 吴珂 刘海舟[1] 刘翟 Ying Cao;Wei Li;Xin Chu;Ke Wu;Haizhou Liu;Di Liu(Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,Hubei,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Bureau of Major R&D Programs,Chinese Academy of Sciences,Beijing 100864,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071 [2]中国科学院微生物研究所,北京100101 [3]中国科学院重大科技任务局,北京100864 [4]中国科学院大学,北京100049

出　　处：《生物工程学报》2020年第5期811-819,共9页Chinese Journal of Biotechnology

基　　金：北京市重大科技专项(No.D171100002217001)资助。

摘　　要：测序技术在通量和成本方面有了较大的改进,以单分子纳米孔测序技术为代表的第三代测序技术更是以其超长读长、实时检测和可以直接检测碱基甲基化修饰等优势在医学及生命科学等领域作出了较大贡献。文中就单分子纳米孔测序技术的原理进行了简要描述,并对其在临床、动物、植物、细菌及病毒等领域的应用和其未来的发展方向进行了讨论。Sequencing technology has been greatly improved in terms of throughput and cost.The single-molecule nanopore DNA sequencing,one of the major branches of the third-generation sequencing technology,has made great contributions in the fields of medicine and life sciences due to its advantages of ultra-long reading length,real-time detection and direct detection of base methylation modification,etc.This article briefly describes the principle of nanopore sequencing technology,and discusses its application in clinical,animal,plant,bacterial and virus fields and its future development direction.

关 键 词：纳米孔测序技术 临床研究 动物基因组 植物基因组 细菌基因组 病毒基因组 

分 类 号：Q811.4[生物学—生物工程]