响应面优化酶促水解鲅鱼蛋白制备ACE抑制肽的研究  

Optimization of enzymatic hydrolysis of ACE inhibitory peptides frommackerel protein by response surface methodology

作　　者：吴若晨 吴正浩 冯译萱 梁晓丽 黄光荣 WU Ruochen;WU Zhenghao;FENG Yixuan;LIANG Xiaoli;HUANG Guangrong(College of life sciences,China jiliang university,Zhejiang Hangzhou 310018,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018

出　　处：《食品工程》2020年第1期28-33,共6页Food Engineering

基　　金：国家大学生创新创业训练计划项目(201810356025)。

摘　　要：以低值小鲅鱼为原料,分别采用胰蛋白酶、碱性蛋白酶、风味蛋白酶等进行水解,以期获得血管紧张素转换酶(ACE)抑制肽。结果表明,碱性蛋白酶水解产物的ACE抑制率最大。通过中心组合响应面设计,对碱性蛋白酶水解工艺条件进行了优化,分析pH值、水解时间、水解温度、酶添加量等因素对水解液ACE抑制率的影响,得到碱性蛋白酶水解制备鲅鱼ACE抑制肽的最优工艺参数为:碱性蛋白酶添加量9981 U/g,水解温度55℃,水解时间4.27 h,水解pH值9.0,此时ACE抑制率可达到46.021%。在该条件下进行验证试验,测得水解液的ACE抑制率为45.5%,与预测值较为相近。In the paper,the low-fat little mackerel was used as the raw material,and trypsin,alkaline protease,and flavor protease were used for hydrolysis to obtain angiotensin-converting enzyme(ACE)inhibitor peptide.The results showed that the ACE inhibition rate of the alkaline protease hydrolysate was the largest.Through the design of the central combination response surface,the alkaline protease hydrolysis process conditions were optimized.The effects of pH,hydrolysis time,hydrolysis temperature,and enzyme addition amount on the ACE inhibition rate of the hydrolysate were analyzed,and the alkaline protease was used to prepare the catfish.The optimal process parameters of ACE inhibitory peptide were:the addition amount of alkaline protease was 9981 U/g,the hydrolysis temperature was 55℃,the hydrolysis time was 4.27 h,and the hydrolysis pHwas 9.0,the ACE inhibition rate can reach 46.021%.A verification test was performed under the condition,and the ACE inhibition rate of the hydrolyzed solution was 45.5%,which was close to the predicted value.

关 键 词：鲅鱼 ACE抑制肽 响应面 酶促水解 蛋白酶 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]