砷和高脂饮食联合暴露对小鼠血清脂联素的影响  

Effects of combined exposure of arsenic and high-fat diet on serum adiponectin in mice

作　　者：原琳 宋选波 纪晓红 李媛媛 赵丽军 Yuan Lin;Song Xuanbo;Ji Xiaohong;Li Yuanyuan;Zhao Lijun(Key Laboratory of Etiology and Epidemiology,National Health Commission,Key Laboratory of Trace Elements and Human Health,Heilongjiang,Institute for Endemic Fluorisis Control,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University,Harbin 150081,China;Department of Endemic Diseases,Sichuan Provincial Center for Disease Control and Prevention,Chengdu 610041,China)

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所,国家卫生健康委病因流行病学重点实验室,黑龙江省微量元素与人类健康重点实验室,150081 [2]四川省疾病预防控制中心地方病预防控制所,成都610041

出　　处：《中华地方病学杂志》2020年第4期248-253,共6页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81773371);黑龙江省自然科学基金(H2016019);黑龙江省博士后基金(LBH-Q17113)。

摘　　要：目的探讨砷和高脂饮食联合暴露对小鼠血清脂联素的影响。方法采用2×3析因设计,将90只雄性C57BL/6小鼠按体重(16~22 g)采用随机数字表法分为6组,分别为标准饮食(STD)对照组、STD+5 mg/L砷组、STD+50 mg/L砷组、高脂饮食(HFD)对照组、HFD+5 mg/L砷组和HFD+50 mg/L砷组,每组15只,在饮水中加亚砷酸钠(NaAsO2),自由饮水、进食。17周后,收集尿样、空腹血样和脂肪组织,原子荧光法检测尿砷含量,血糖仪检测血糖含量,试剂盒酶法检测血脂含量[包括血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL)-c],酶联免疫吸附试验检测血清胰岛素、总脂联素和高分子量(HMW)脂联素含量。结果砷暴露和HFD对小鼠血糖、血脂含量无交互作用(P均>0.05),对血清胰岛素和总脂联素含量存在交互作用(P均<0.05)。HFD可以显著增加小鼠血糖、血清TC含量(P均<0.05),而血清TG和HDL-c含量未见明显改变(P均>0.05)。另外,STD+50 mg/L砷组血清TG和HDL-c含量显著低于STD对照组(mmol/L:0.72±0.14比0.88±0.24,0.67±0.03比0.80±0.16,P均<0.05)。与STD对照组比较,HFD对照组和STD+5、50 mg/L砷组血清胰岛素含量差异无统计学意义(P均>0.05),而HFD+5、50 mg/L砷组血清胰岛素含量显著下降(mU/L:14.71±4.16比11.42±0.78、11.52±1.53,P均<0.05);与STD对照组比较,HFD对照组和STD+5、50 mg/L砷组血清总脂联素、HMW脂联素含量和HMW脂联素与总脂联素比值显著降低(P均<0.05),而HFD+5 mg/L砷组上述3种脂联素指标显著高于HFD对照组(P均<0.05)。STD时,经对数转换的小鼠尿砷含量与血清总脂联素和HMW脂联素含量间呈显著负相关[偏相关系数(r)=-0.549、-0.608,P均<0.01]。结论砷暴露和HFD均可以使小鼠糖脂代谢发生改变,但二者的表现形式不同;两者可协同降低血清胰岛素含量,对血清脂联素含量有拮抗作用。Objective To investigate the effects of combined exposure of arsenic and high-fat diet(HFD)on serum adiponectin in mice.Methods According to the 2×3 factorial design,a total of 90 male C57BL/6 mice were randomly divided into 6 groups using random number table method based on body weight(16-22 g):standard diet(STD)control group,STD+5 mg/L arsenic group,STD+50 mg/L arsenic group,HFD control group,HFD+5 mg/L arsenic group and HFD+50 mg/L arsenic group.There were 15 mice in each group,and sodium arsenite(NaAsO2)was added to the drinking water.Mice were accessed freely to water and fed ad libitum.After 17 weeks,urine samples,fasting blood samples and adipose tissue were collected.Urinary arsenic was determined by atomic fluorescence.Blood glucose meter was used to measure blood glucose.Levels of blood lipid contents,including serum triglyceride(TG),total cholesterol(TC),and high density lipoprotein cholesterol(HDL)-c,were examined by kit enzymatic method.Levels of insulin,total adiponectin and high molecular weight(HMW)adiponectin were examined by enzyme-linked immunosorbent assay.Results There was no interaction between arsenic exposure and HFD on the effects of blood glucose and blood lipids(P>0.05).There was an interaction between these two factors on serum insulin and total adiponectin(P<0.05).HFD can significantly increase blood glucose,serum TC levels(P<0.05),but not TG and HDL-c in mice(P>0.05).The levels of TG and HDL-c in STD+50 mg/L arsenic group were significantly decreased as compared to those of STD control group(mmol/L:0.72±0.14 vs 0.88±0.24,0.67±0.03 vs 0.80±0.16,P<0.05).Compared with STD control group,there was no significant difference in serum insulin level in HFD control group and STD+5 or 50 mg/L arsenic groups(P>0.05),but insulin levels in HFD+5 or 50 mg/L arsenic groups were significantly decreased(mU/L:14.71±4.16 vs 11.42±0.78,11.52±1.53,P<0.05).Compared with STD control group,serum total adiponectin,HMW adiponectin levels,and the ratio of HMW adiponectin to total adiponectin were signif

关 键 词：亚砷酸盐类 高脂饮食 胰岛素 总脂联素 高分子量脂联素 

分 类 号：R15[医药卫生—营养与食品卫生学]