2种高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的不确定度评定  

Uncertainty evaluation for the determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph

作　　者：董靖 张潇 刘越 李尔春 李卓 张亚锋 郭端 张凡 DONG Jing;ZHANG Xiao;LIU Yue;LI Er-Chun;LI Zhuo;ZHANG Ya-Feng;GUO Duan;ZHANG Fan(XT'an Institute for Food and Drug Control,Xi'an 710054,China)

机构地区：[1]西安市食品药品检验所,西安710054

出　　处：《食品安全质量检测学报》2020年第7期2190-2196,共7页Journal of Food Safety and Quality

基　　金：食品安全风险物质筛查检测服务平台建设(2019PT-22)。

摘　　要：目的评定并比较2种厂家高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的不确定度。方法分别采用Agilent1260型及Thermo U3000型高效液相色谱仪测定芝麻酱中黄曲霉毒素B1的含量,应用不确定度理论分析检测数学模型,对测定不确定度的来源进行分析和量化。结果结果表明当黄曲霉毒素B1的测定结果(Agilent 1260、Thermo U3000)分别为6.1236、6.0230μg/kg时,其扩展不确定度分别为0.42706、0.31115μg/kg,k=2。结论标准溶液的配制、由最小二乘法拟合标准曲线计算样品浓度以及回收率测定引入的不确定度对结果影响较大。在本实验条件下,Agilent1260型测定引入的相对不确定度大于Thermo U3000型测定引入的不确定度,后者仪器更加稳定,结果更准确。Objective To evaluate and compare the difference of uncertainty of determination of aflatoxin B1 in sesame sauce by 2 kinds of high performance liquid chromatograph.Methods The content of aflatoxin B1 in sesame sauce was determined by Agilent 1260 and Thermo U3000 high performance liquid chromatography,and the mathematical model of uncertainty analysis was used to analyze and quantify the sources of measurement uncertainty.Results The results(Agilent 1260,Thermo U3000)showed when the determination results of aflatoxin B1 were 6.1236μg/kg and 6.0230μg/kg,the extended uncertainties were 0.42706μg/kg,0.31115μg/kg,and k=2.Conclusion The uncertainty introduced by the preparation of standard solution,the calculation of sample concentration by the least square method fitting standard curve and the determination of recovery has great influence on the results.Under this experimental condition,the relative uncertainty introduced by the Agilent 1260 measurement is greater than that introduced by the Thermo U3000 measurement,which is more stable and more accurate.

关 键 词：黄曲霉毒素B1 高效液相色谱法 不确定度 

分 类 号：O657.72[理学—分析化学] TS229[理学—化学]