副干酪乳杆菌的发酵条件优化及其发酵液功效评价  

Optimization of fermentation conditions of Lactobacillusparacasei and evaluation of its efficacy

作　　者：苏艳凤 陈新慧 李萌[1] 赵丹[1] 张佳婵[1] 王昌涛[1,2] SU Yan-feng;CHEN Xin-hui;LI Meng;ZHAO Dan;ZHANG Jia-chan;WANG Chang-tao(Key Laboratory of Plant Resources Research and Development,College of Science,Beijing Technology and Business University,Beijing 100083,China;Beijing Technology and Business University,Center for Advanced Innovation in Food Nutrition and Human Health,Beijing 100083,China)

机构地区：[1]北京工商大学理学院植物资源研究与开发重点实验室,北京100083 [2]北京工商大学食品营养与人类健康高精尖创新中心,北京100083

出　　处：《日用化学工业》2020年第6期402-407,426,共7页China Surfactant Detergent & Cosmetics

摘　　要：为开发副干酪乳杆菌发酵产物在化妆品领域的应用,对副干酪乳杆菌的培养条件运用单因素实验进行优化并测定副干酪乳杆菌发酵液中多肽和总糖的含量。对副干酪乳杆菌发酵液进行DPPH自由基、超氧阴离子自由基清除率实验和ABTS·+抗氧化能力测定。用HaCat细胞建立UV损伤细胞模型,采用Cusabio人白介素10(IL-10)和人组织蛋白酶B(CTSB)酶联免疫试剂盒进行检测。并测定副干酪乳杆菌发酵液对HaCat细胞溶酶体活性的影响。抗氧化实验数据结果表明副干酪乳杆菌发酵液具有DPPH自由基、超氧阴离子自由基清除能力和ABTS·^+抗氧化能力。5%的副干酪乳杆菌发酵液能使IL-10表达量较模型组下降18 pg/mL,说明副干酪乳杆菌发酵液具有抗UV引起的免疫抑制的功效。10%的副干酪乳杆菌发酵液对CTSB表达的抑制作用与阳性对照组(乙酰水杨酸)相当,说明10%的副干酪乳杆菌发酵液抗炎效果与5 mmol/L乙酰水杨酸相当。在5%副干酪乳杆菌发酵液作用下,溶酶体活性较模型组提高23%,进一步说明副干酪乳杆菌发酵液具有良好的抗炎效果。In order to develop the application of Lactobacillus paracasei fermentation products in cosmetics,the culture conditions of Lactobacillus paracasei were optimized by single factor experiment and the content of polypeptide and total sugar in Lactobacillus paracasei fermentation broth were determined.DPPH,superoxide anion clearance test and ABTS·^+antioxidant capacity were measured on Lactobacillus paracasei fermentation.A UV-injured cell model was established using HaCat cells and detected using Cusabio human interleukin 10(IL-10)and human cathepsin B(CTSB)enzyme-linked immunosorbent kits.The effects of Lactobacillus paracasei fermentation broth on the lysosomal activity of HaCat cells were determined.The results of antioxidant data showed that the Lactobacillus paracasei fermentation broth had scavenging ability for DPPH free radicals and superoxide anions,and antioxidant capacity for ABTS·+.5%Lactobacillus paracasei fermentation broth could reduce IL-10 expression by 18 pg/mL compared with the model group,indicating that Lactobacillus paracasei fermentation broth had anti-UV-induced immunosuppressive effect.The inhibitory effect of 10%Lactobacillus paracasei fermentation broth on CTSB expression was comparable to that of the positive control group(aspirin),indicating that the anti-inflammatory effect of 10%Lactobacillus paracasei fermentation broth was comparable to that of 5 mmol/L aspirin.In the presence of 5%Lactobacillus paracasei fermentation broth,the lysosomal activity was increased by 23%compared with the model group,further indicating its good anti-inflammatory effect.

关 键 词：副干酪乳杆菌 优化 发酵液 抗炎 抗氧化 化妆品添加剂 

分 类 号：TQ658[化学工程—精细化工]