Neuroprotective effect of chitosan nanoparticle gene delivery system grafted with acteoside (ACT) in Parkinson's disease models  

作　　者：Yongyong Xue Na Wang Zhi Zeng Jinpeng Huang Zhiming Xiang Yan-Qing Guan 

机构地区：[1]MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou,510631,China [2]School of Life Science,South China Normal University,Guangzhou,510631,China [3]South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research,Panyu Central Hospital,Guangzhou,China [4]Department of Radiology,Guangzhou Panyu Center Hospital,Guangzhou,511400,China

出　　处：《Journal of Materials Science & Technology》2020年第8期197-207,共11页材料科学技术（英文版）

基　　金：supported financially by the National Natural Science Foundation of China(Nos.31370967 and 31170919);the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2014);the Science and Technology Planning Project of Guangdong Province(No.2015A020212033);the Science and Technology Project of Guangzhou(No.201805010002);the Innovation Project of Graduate School of South China Normal University(No.2018LKXM019)。

摘　　要：Developing new drugs to treat Parkinson's disease efficiently is challenging. Here we report that chitosan nanoparticles(APPDNs) could serve as novel candidates for the design of anti-PD drugs. In this study, we investigated the effects of chitosan poly ethyleneglycol-poly lactic acid(PEG-PLA) nanoparticles conjugated with nerve growth factor(NGF), acteoside(ACT) and plasmid DNA(p DNA) for PD therapy using in vitro and in vivo models. Using PD cell models, we demonstrated that APPDN had good neuroprotective effects. More significantly, experiments using mouse PD models demonstrated that APPDNs could ameliorate the behavioral disorders of sick mice. Immunohistochemical and western blot(WB) analyses demonstrated that APPDNs could significantly reverse dopaminergic(DA) neuron loss in the substantia nigra and striatum of sick mice. This study opens up a novel avenue to develop anti-PD drugs.

关 键 词：Parkinson's disease Chitosan nanoparticles ACTEOSIDE Gene therapy PDNA 

分 类 号：R742.5[医药卫生—神经病学与精神病学]