微球菌Micrococcus NT_1001解磷特性研究  

Study on the Phosphorus Dissolving Characteristics of Micrococcus NT_1001

作　　者：蒋学剑 王志强 王志清 何珣 陈可泉[2] JIANG Xue-jian;WANG Zhi-qiang;WANG Zhi-qing(Tanggou Liangxianghe Liquor Co.,Ltd.,Lianyungang,Jiangsu 222535;State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology Pharmaceutical Engineering,Nanjing Tech University,Nanjing,Jiangsu 211816)

机构地区：[1]江苏汤沟两相和酒业有限公司,江苏连云港222535 [2]材料化学工程国家重点实验室,南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《安徽农业科学》2020年第12期168-172,共5页Journal of Anhui Agricultural Sciences

基　　金：江苏省博士后科研资助计划(2019K242)。

摘　　要：从汤沟酒厂的酒醅样品中分离得到一株解磷细菌,为革兰氏阳性(G+)菌,菌落表面呈金黄色,且形态圆整,边缘整齐,成堆排列。经16S rDNA序列比对,该菌株为微球菌,被命名为Micrococcus NT_1001。分别以KH 2PO 4和蛋黄卵磷脂(PC)为底物,考察Micrococcus NT_1001对无机磷和有机磷的分解性能。结果表明,Micrococcus NT_1001可降解无机磷和有机磷,且可转化PC生产甘油磷酸胆碱(GPC);培养温度、pH和培养基中的初始磷浓度对磷分解率、GPC收率有显著影响;Micrococcus NT_1001的最适生长条件为37℃,pH为8.0;初始KH2PO4浓度为60 mg/L时,KH2PO4的分解率最高,为97.89%;初始PC浓度为10 g/L时,GPC收率最高,为1.33%;KH 2PO 4和PC都对Micrococcus NT_1001存在底物抑制作用。A wild gram-positive(G+)phosphate-dissolving bacteria was isolated from the fermented grains samples of Tanggou Distillery.The colony surface was golden yellow,and the colony shape was round.The colony edges were neat and arranged in piles.The aligned 16S rDNA sequence indicated that this strain belonged to Micrococcus sp,which was named as Micrococcus NT_1001.The decomposition performance of Micrococcus NT_1001 on inorganic phosphorus and organic phosphorus was investigated separately by using KH 2PO 4 and egg yolk lecithin(PC)as substrate.The results showed that Micrococcus NT_1001 could degrade effectively inorganic phosphorus and organic phosphorus,and also convert PC to glycerol phosphate choline(GPC);culture temperature,pH and initial phosphorus concentration in the medium had significant effects on the phosphorus decomposition rates and GPC yields;the optimal growth condition of Micrococcus NT_1001 was 37℃,pH 8.0;when the initial concentration of KH2PO4 was 60 mg/L,the highest decomposition rate of KH2PO4 was obtained,the corresponding value was 97.89%;when the initial PC concentration was 10 g/L,the highest GPC yield(1.33%)was obtained;both KH 2PO 4 and PC had substrate inhibitory effects on Micrococcus NT_1001.

关 键 词：酒醅 微球菌 解磷 PC GPC 底物抑制 

分 类 号：TS201.3[轻工技术与工程—食品科学]