副溶血弧菌Ⅵ型分泌系统对其种内竞争的影响  

Effect of Vibrio parahaemolyticus T6SS on intraspecific competition

作　　者：穆丽丽 牛犇 张昭寰 王思琦 王敬敬[1] 刘海泉[1,2,3,4] 潘迎捷 赵勇[1,2,3] MU Lili;NIU Ben;ZHANG Zhaohuan;WANG Siqi;WANG Jingjing;LIU Haiquan;PAN Yingjie;ZHAO Yong(College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;laboratory of Qualiy and Safety Risk Asessment for Aquatic Product on Storage and Preservation,Ministry of Agriculture and Rural Affairs,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaie Product Proessing&Preseration,Shanghai 201306,China;Engineering Research Center of Food Thermal Processng Technology,Shanghai Oeean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]农业农村部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [3]上海水产品加工及贮藏工程技术研究中心,上海201306 [4]上海海洋大学食品热加工工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2020年第3期429-438,共10页Journal of Shanghai Ocean University

基　　金：国家自然科学基金面上项目(31671779,31571917);国家重点研发计划(2018YFC1602205);上海市科技兴农项目(沪农科攻字2016第1-1号,沪农科推字2017第4-4号);上海市教育委员会科研创新计划(2017-01-07-00-10-E00056);上海市教委曙光计划(15SG48)

摘　　要：对85株副溶血弧菌的Ⅵ型分泌系统(type Ⅵsecretion system,T6SS)相关基因的携带情况进行分析,并选取部分副溶血弧菌菌株进行混合培养,探究其种内竞争情况。利用qPCR技术,分析混合培养后T6SS相关基因的表达情况。结果表明:85株副溶血弧菌均携带T6SS2,62株副溶血弧菌携带T6SS1,其中临床分离株41株,占全部临床分离株的93.2%;环境分离株21株,占全部临床分离株的51.2%,并且T6SS1基因簇在不同来源的副溶血弧菌中存在一定的差异性。在混合培养条件下,部分T6SS1^+的副溶血弧菌存在明显的种内竞争优势。基因表达分析结果显示,较单一培养的菌株,混合培养中副溶血弧菌T6SS1和T6SS2相关基因的表达量均有明显的上调。导致这一现象的原因可能是在混合培养的条件下,副溶血弧菌可通过增强自身T6SS1和T6SS2相关基因的表达,以提升其在种内的竞争力。初步探究了副溶血弧菌Ⅵ型分泌系统对其种内竞争的影响,为副溶血弧菌T6SS功能的进一步揭示提供科学的参考。The 85 strains of Vibrio parahaemolyticus harboring the Vibrio parahaemolyticus type Ⅵ secretory system(T6 SS) related genes were investigated in this study. The T6 SS1^+ and T6 SS1^- strains chosen were analysed in mixed culture to explore their intraspecific competition. The qPCR technology was employed to analyze the expression of related genes of T6 SS. The results show that: although all Vibrio parahaemolyticus harbored T6 SS2, T6 SS1 were found in a certain number of strains, 85 strains of Vibrio parahaemolyticus all carried T6 SS2, and 62 strains of Vibrio parahaemolyticus carried T6 SS1, of which 41 were clinical isolates, accounting for 93.2% of all clinical isolates;there were 21 environmental isolates, accounting for 51.2% of all environmental isolates, T6 SS1 gene clusters were different in Vibrio parahaemolyticus from different sources. Some T6 SS1^+ strains had significant intraspecific competitive advantages, which could suppress the growth of other strains in mixed culture. Compared with monoculture strains, the expression levels of T6 SS1 and T6 SS2 strains were significantly up-regulated in mixed culture. The reason for this phenomenon might be that strains could strengthen the expression of its related T6 SS1 and T6 SS2 genes to enhance its competitiveness in the species. The influence of Vibrio parahaemolyticus type Ⅵ secretion system on its intraspecies competition was preliminarily explored, providing scientific reference for further revealing Vibrio parahaemolyticus T6 SS function.

关 键 词：副溶血弧菌 T6SS 种内竞争 定量PCR 基因表达 

分 类 号：TS201.3[轻工技术与工程—食品科学]