白花丹素调控ROS抑制NLRP3炎症小体减轻IgA肾损伤机制的实验研究  

Effect and mechanism of plumbagin on reducing podocyte injury in rats with IgA nephropathy by regulating ROS and inhibiting NLRP3

作　　者：刘昌明[1] 黄香尘 杜斌[1] LIU Chang-ming;HUANG Xiang-chen;DU Bin(Pathological Staff Room,Ya'an Vocational and Technical College,Ya'an 625000,Sichuan Province,China;Department of Pathology,Affiliated Hospital of Ya'an Vocational and Technical College,Ya'an 625000,Sichuan,Province,China)

机构地区：[1]雅安职业技术学院病理教研室,四川雅安625000 [2]雅安职业技术学院附属医院病理科,四川雅安625000

出　　处：《中国临床解剖学杂志》2020年第3期308-313,共6页Chinese Journal of Clinical Anatomy

基　　金：四川省医学青年科研计划项目(Q15008)。

摘　　要：目的研究白花丹素对IgA肾病的作用和机制。方法按Ying等改良的BSA+LPS+CCl4方法复制实验IgA肾病模型;然后,给药组大鼠每组分别腹腔注射10 mg/kg、20 mg/kg和50 mg/kg白花丹素,1次/d,对照组和模型组腹腔注射等量的生理盐水1次/d;8周后,全自动化学分析仪检测24 h尿蛋白、血肌酐、血尿素,流式检测ROS含量,试剂盒检测SOD活性和MDA含量,HE染色观察病理损伤,Elisa检测TNF-α、IL-18、IL-1β,蛋白印迹法检测NLRP3、ASC、caspase-1 p20、P13K、AKT和NF-kB蛋白表达。结果与模型组相比,给药组大鼠的尿蛋白、血清肌酸酐和尿素氮含量显著减少,ROS水平显著降低,SOD含量显著增多,MDA含量显著减少,MDA、IL-1β、IL-18和TNF-α的含量显著减少,NLRP3、ASC、caspase-1 p20、P13K、AKT和NF-kB的蛋白表达显著下调,并且随着给药量的增加效果越显著。结论白花丹素通过降低尿蛋白、血肌酐和血尿素含量,减轻病理损伤,抑制氧化应激、炎症反应和NLRP3/P13K/AKT/NF-kB通路激活来减轻IgA肾病。Objective To investigate the role and mechanism of plumbagin in IgA nephropathy.Methods First,the IgA nephropathy model was constructed according to the modified BSA+LPS+CCl4 method of Ying et al.Then,the rats in the administration group were intraperitoneally injected with 10 mg/kg,20 mg/kg or 50 mg/kg of plumbagin per day.The normal group and the model group were intraperitoneally injected with the same amount of normal saline per day,after 8 weeks later,24 h urine protein,serum creatinine,and blood urea were detected by a fully automatic chemical analyzer,and the ROS content was detected by flow,and the SOD activity and MDA content were detected.Pathological damage was observed by HE staining,TNF-a,IL-18,IL-1βwere detected by Elisa,and NLRP3,ASC,caspase-1 p20,P13 K,protein expression of AKT and NF-kB were detected by Western blotting.Results Compared with the model group,the proteinuria,serum creatinine and urea nitrogen levels of the administration group significantly reduced,ROS levels significantly decreased,SOD levels increased,MDA,IL-1 b,IL-18 and TNF-a levels were significantly increased.Protein expression of NLRP3,ASC,caspase-1 p20,P13 K,AKT and NF-kB was significantly down-regulated,with the increasing of dose,the effect was more pronounced.Conclusions Plumbagin mainly reduces IgA nephropathy by reducing urinary protein,serum creatinine and blood urea,reducing pathological damage,inhibiting oxidative stress,inflammatory response and activation of NLRP3/P13 K/AKT/NF-kB pathway.

关 键 词：白花丹素 IGA肾病 ROS NLRP3 

分 类 号：R734.2[医药卫生—肿瘤]