生姜提取物对副溶血弧菌抑制作用研究  

A study on the antibacterial activity of the extracts of Zingiber Officinale Roscoe against Vibro parahaemolyticus

作　　者：张政 马更勤 王宏勋 侯温甫 周敏 ZHANG Zheng;MA Geng-qin;WANG Hong-xun;HU Wen-fa;ZHU Min(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Hubei Provincial Key Laboratory for Agricultural Products Processing and Conversion,WuHan 430023,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,湖北武汉430023 [2]湖北省农产品加工与转化重点实验室,湖北武汉430023

出　　处：《武汉轻工大学学报》2020年第2期1-6,21,共7页Journal of Wuhan Polytechnic University

基　　金：湖北省教育厅科学技术研究项目重点项目(D20161702).

摘　　要：本文研究了以乙醇作为提取剂、提取温度60℃、提取时间60 min的条件下,不同提取方法对提取生姜中活性成分提取率的影响。实验结果表明,超声波提取法的提取率最高。同时,本研究还测定了生姜提取物对副溶血弧菌的最低抑菌浓度(MIC)与最低杀菌浓度(MBC),以量化生姜提取物对副溶血弧菌的抑制作用。同时研究了pH值、浓度、紫外线照射处理、温度等条件对生姜提取物抑菌效果的影响。实验表明,生姜提取物对副溶血弧菌有明显的抑制作用,其MIC、MBC值均为40 mg/mL,结果显示:pH值、浓度、紫外线照射处理、温度对生姜提取物抑菌效果有显著的影响。To study the effects of different extraction methods on the extraction rate of effective components from Zingiber officinale Roscoe under the conditions of ethanol as extraction agent,extraction temperature is 60℃and extraction time is 60min.The results showed that The results the optimum method was ultrasonic extraction.At the same time,minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of extraction of Zingiber officinale Roscoe to vibrio parahaemolyticus were also determined in this study to quantify the inhibitory effect of extraction of Zingiber officinale Roscoe to vibrio parahaemolyticus.Effects of pH,concentration,uv irradiation and temperature on bacteriostasis of extraction were studied.The results showed that extraction had a significant inhibitory effect on vibrio parahaemolyticus,and the MIC and MBC values were both 40 mg/mL.The results also showed that pH,concentration,uv irradiation and temperature had significant effects on the antibacterial effect of extraction.

关 键 词：生姜提取物 副溶血弧菌 抑菌作用 超声波提取法 

分 类 号：R155.5[医药卫生—营养与食品卫生学]