Study on the Effects of Reducing Blood Lipid by Flavonoids from Ampelopsis grossedentata on Hyperlipidemia Rats  

藤茶黄酮对高脂大鼠的降血脂作用研究

作　　者：ZUO Xue-mei LIU Qian LIAO Fang-fan FU Ming LI Sheng-hua XIAO Qiang 左雪媚;刘倩;廖芳帆;付明;李胜华;肖强(怀化学院生物与食品工程学院,民族药用植物资源研究与利用湖南省重点实验室,湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008)

机构地区：[1]College of Biological and Food Engineering,Huaihua University,Hunan Provincial Key Laboratory of Research and Utilization of Ethnic Medicinal Plant Resources,Key Laboratory of Medicinal Plants and Ethnobotany in Western Hunan Province,Huaihua 418008,PRC

出　　处：《Agricultural Science & Technology》2020年第1期33-40,共8页农业科学与技术（英文版）

基　　金：湖南省双一流学科建设经费资助;湖南省高校创新平台开放基金项目(14K075);民族药用植物资源研究与利用湖南省重点实验室开放基金项目(YYZW2015-1)。

摘　　要：In order to study the effects of Ampelopsis grossedentata on endogenous cholesterol biosynthesis and the effects of reducing blood lipid in hyperlipidemia rats,extraction of flavonoids from Ampelopsis grossedentata by using ethanol(TFAG),and the effects of TFAG on intracellular cholesterol synthesis were detected by amphotericin B-cell model;SD hyperlipidemia rat model was established by feeding high fat diet.A formulated medicine called Xuezhikang was used as a positive control,and TFAG of different doses were administered to the stomach for 30 d continuously to measure the indexes of heart,liver tissue homogenate and serum;part of the liver was taken for pathological observation.The results showed that TFAG could significantly inhibit the synthesis of cholesterol in cells.TFAG of different doses could significantly reduce the content of TC and TG in serum of hyperlipidemia rats,and increase the content of HDL;TC and TG in heart and liver were also decreased;besides,it could increase the content of SOD,CAT and GSH in the liver of hyperlipidemia rats,and reduce the content of MDA.The results of pathological section showed that TFAG could improve the damage degree of hepatocytes in hyperlipidemia rats,and the effect of high dose group was similar to that of Xuezhikang group.In general,TFAG has good antioxidant and reducing blood lipid effects,and can effectively inhibit liver steatosis.为了探讨藤茶对细胞内源性胆固醇生物合成的影响以及对高脂血症大鼠的降血脂效果,采用乙醇提取藤茶黄酮(TFAG),用两性霉素B-细胞模型检测其对细胞内胆固醇合成的影响;通过喂食高脂饲料建立SD高脂大鼠模型,以血脂康为阳性对照,连续灌胃不同剂量的TFAG 30 d,测心、肝组织匀浆和血清中的各项指标;取部分肝脏进行病理学观察。结果表明:TFAG能明显抑制细胞内胆固醇的合成,不同剂量的TFAG能明显降低高脂大鼠血清中的TC、TG含量,增加HDL的含量;对心、肝组织中的TC、TG也有明显降低作用;能提高高脂大鼠肝脏中SOD、CAT和GSH的含量,降低MDA的含量。病理切片结果显示TFAG能改善高脂大鼠模型肝细胞的受损程度,其中高剂量组的效果与血脂康组相当。总体而言,TFAG具有较好的抗氧化和降血脂效果,并能有效抑制肝脏脂肪变性。

关 键 词：Flavonoids from Ampelopsis grossedentata RAT HYPERLIPIDEMIA Cholesterol biosynthesis Reducing blood lipid 

分 类 号：R285.5[医药卫生—中药学]