偶发流产和复发流产组织的基因芯片检测结果比较  

Comparison of gene chip detection results between spontaneous abortion and recurrent abortion

作　　者：石铭 张远 张静[3] 王澍 刘伶 邵小光 Shi Ming;Zhang Yuan;Zhang Jing;Wang Shu;Liu ling;Shao Xiaoguang(Reproductive and Genetic Medicine Center,Dalian Municipal Women and Children′s Medical Center,Dalian 116037,China;Dalian Medical University,Dalian 116044,China;Department of Laboratory,Dalian Central Hospital,Dalian 116033,China;Department of Neurosurgery,Sanbo Brain Hospital,Capital Medical University,Beijing 100093,China;Department of Pediatric Hematology-Oncology,Dalian Municipal Women and Children′s Medical Center,Dalian 116037,China)

机构地区：[1]大连市妇女儿童医疗中心生殖与遗传医学中心,116037 [2]大连医科大学,116044 [3]大连市中心医院检验科,116033 [4]首都医科大学三博脑科医院神经外科,北京100093 [5]大连市妇女儿童医疗中心儿童血液肿瘤科,116037

出　　处：《中国医师进修杂志》2020年第6期496-499,共4页Chinese Journal of Postgraduates of Medicine

基　　金：辽宁省科学技术基金(2019-ZD-0877)。

摘　　要：目的使用微阵列比较基因组杂交技术,分析自然流产组织的染色体核型,探讨偶发流产和复发流产的遗传学差异,提供遗传学指导。方法收集2016年7月至2019年9月就诊于大连市妇女儿童医疗中心确诊为自然流产的患者,对于符合纳入标准的患者进行流产组织基因芯片检测分析。结果共收集病例618例,其中345例符合纳入标准,偶发流产133例(38.55%),复发流产212例(61.45%)。共检测出异常染色体237例,其中22号三体型(10.14%)、16号三体型(9.86%)、19号单体型(7.25%)发生率较高。复发流产组中的染色体核型异常率更低,但两组染色体核型的异常率比较差异无统计学意义(P>0.05);经过不同年龄和孕周亚组分析后,单体型和三体型的染色体畸形发生率比较差异无统计学意义(P>0.05)。结论部分核型异常在流产组织中较为常见,在复发性流产组织中,染色体核型的异常率更低,但偶发流产和复发流产患者的遗传学背景无显著差异。Objective By using array-based comparative genomic hybridzation(CGH),the karyotype of spontaneous abortion was detected to analyze the genetic background difference between initial abortion and recurrent abortion.Methods From July 2016 to September 2019,patients diagnosed as spontaneous abortion in Dalian Women′s and Children′s Medical Center were collected continuously.The abortion tissue microarray was used to analyze.Results A total of 618 cases were collected,345 of whom met the inclusion criteria,133 cases(38.55%)in spontaneous abortion group and 212 cases(61.45%)in recurrent abortion group.A total of 237 cases of abnormal chromosome were detected,among whom the incidence of trisomy 22(10.14%),trisomy 16(9.86%)and monosomy 19(7.25%)was high.The abnormal rate of chromosome karyotype was lower in recurrent abortion group,but there was no significant statistical difference(P>0.05).After analyzing different age and gestational age,there was no significant statistical difference between haplotype and trisomy(P>0.05).Conclusions Some abnormal karyotypes are more common in abortion tissues.In recurrent abortion,the abnormal rate of chromosome karyotype is lower,but there is no significant difference in genetic background between spontaneous abortion and recurrent abortion.

关 键 词：微阵列分析 流产 自然 基因测定 流产 习惯性 偶发流产 

分 类 号：R440[医药卫生—诊断学] R714.21[医药卫生—临床医学]