Immobilization of β-glucuronidase:biocatalysis of glycyrrhizin to 18β-glycyrrhetinic acid and in-silico lead finding  

作　　者：Makhmur Ahmad Mohammad Rashid Babar Ali Shamshir Khan Naseem Akhtar Mohd Faiyaz Khan Bibhu Prasad Panda 

机构地区：[1]Department of Pharmaceutics,College of Dentistry and Pharmacy,Buraydah,Al-Qassim 31717,Kingdom of Saudi Arabia [2]Department of Pharmacognosy and Medicinal Chemistry,College of Dentistry and Pharmacy,Buraydah,Al-Qassim 31717,Kingdom of Saudi Arabia [3]Department of Clinical Pharmacy,Prince Sattam bin Abdul Azeez University,Al-Kharj,Kingdom of Saudi Arabia [4]Microbial and Pharmaceutical Biotechnology L aboratory,School of Pharmaceutical Education and Research,Center for Advanced Research in Pharmaceutical Sciences,Jamia Hamdard,New Delhi-110062,India

出　　处：《Journal of Chinese Pharmaceutical Sciences》2020年第5期333-340,共8页中国药学（英文版）

摘　　要：The non-covalent immobilization ofβ-glucuronidase enzyme obtained from Rhizopus oryzae was carried out by entrapment in natural fiber(papaya and coconut).The bioconversion capability of immobilized enzyme was analyzed based on conversion of glycyrrhizin to 18β-glycyrrhetinic acid under different conditions.The hydrolytic activity of theβ-glucuronidase enzyme was highly depended on the microbial source and matrix,in which enzyme was immobilized.R.oryzaeβ-glucuronidase immobilized in papaya fibers produced the highest GA content(13.170μg/mL)at 10 h of reaction.However R.oryzaeβ-glucuronidase immobilized in coconut fibers produced the highest GA content(21.425μg/mL)at 15 h of reaction.Online Molinspiration software was used to predict drug like molecular properties of the 18β-glycyrrhetinic acid,and software suggested that the compounds had potential of becoming the orally active molecules.Therefore,in silico studies were conducted on proposed 18β-glycyrrhetinic acid to select the best possible drug candidates based on drug properties and bioactivity score of the compounds.

关 键 词：18β-Glycyrrhetinic acid GLYCYRRHIZIN Β-GLUCURONIDASE Natural fiber In-silico study 

分 类 号：R284[医药卫生—中药学]