相关期刊:《Journal of Ocean University of China》《Science China(Life Sciences)》《World Journal of Gastroenterology》《Journal of Biomedical Science and Engineering》更多>>
supported by the National Natural Science Foundation of China(No.22177040);partly funded by the Fundamental Research Funds for the Central Universities(No.JUSRP123037);the 111 Project(No.111-2-06).
We report the design and development of aβ-glucuronidase(β-Glu)-responsive ManNAz derivative,Glu-AAM,for tumor-selective metabolic glycoengineering.Glu-AAM enables specific labeling of tumor cell surface sialoglycan...
With increasing evidence,the biliary tract and the gallbladder mucosa are no longer considered sterile environments devoid of bacteria.Rather a profound biofilm of resident bacterial flora is associated with the mucos...
supported by the National Natural Science Foundation of China(81801858);China Postdoctoral Science Foundation(2018M641676);the Applied Basic Research Program of Shanxi Province(201801D221408)。
β-Glucuronidase(GUS)plays a key role in tumor initiation,metastasis,and progression,and thus,has been proposed as a promising cancer biomarker.In this study,we designed an enzyme-activatable near-infrared(NIR)fluores...
funded by the National Key Research and Development Program of China(Grant No.2016YFD0100601-15);Zhejiang Fundamental Public Welfare Research Program(Grant No.LGN19C140008);Zhejiang Provincial Key Research and Development Plan(Grant No.2019C02006);Major Scientific and Technological Innovation 2025 Project of Ningbo(Grant No.2019B10004);Major Agricultural Science and Technology Project in Ningbo(Grant No.2016C11017).
OsPRIOa is one of the well known pathogenesis-related genes in rice,and is induced by multiple plant hormones and pathogens.However,the underlying transcriptional regulation mechanisms in response to differe nt signal...
A method for transient gene expression was developed for western white pine(WWP,Pinus monticola Dougl.ex D.Don)using reporter gene uidA encodingβ-glucuronidase(GUS).GUS was transiently expressed in cross sections of ...
The non-covalent immobilization ofβ-glucuronidase enzyme obtained from Rhizopus oryzae was carried out by entrapment in natural fiber(papaya and coconut).The bioconversion capability of immobilized enzyme was analyze...
Project supported by the National Natural Science Foundation of China(Nos.31772213 and 31972320);the Excellent Youth Fund of Zhejiang Province,China(No.LR17C140002)
An efficient genetic transformation system and suitable promoters are essential prerequisites for gene expression studies and genetic engineering in streptomycetes.In this study,firstly,a genetic transformation system...
Acknowledgements This work was financially supported by the National Natural Science Foundation of China (Grant. Nos. 21506011 and 21425624), and China Postdoctoral Science Foundation funded project (No. 2015M570038).
β-Glucuronidase from Penicillium purpuro- genum Li-3 (PGUS) can efficiently hydrolyze glycyrrhizin into the more valuable glycyrrhetic acid monoglucuronide. However, a low productivity of PGUS and the lack of an ef...
supported by grants from Shandong Provincial Natural Science Foundation (ZR2011CQ016 to Shijuan Liu);National Natural Science Foundation of China (31200196 to Shijuan Liu and 31170236 to Wei Zhang)
Bidirectional promoters are relatively abundant in eukaryotic genomes, suggesting that they have an important biological significance. As yet, few of these promoters have been characterized in detail. Here, using a pr...
Mesoporous silica particles were prepared for efficient immobilization of the β-glucuronidase (GUS) through a biomimetic mineralization process, in which the solution containing lysozyme and GUS were added into the...