Rapid Quantitative Determination of Isoprene Monomer in Living Taraxacum kok-saghyz by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry  

作　　者：Xiang Tong Guo Tianyang Zhang Xi Chen Yunhan Dong Yiyang Zhang Jichuan Ma Qiang Zhang Liqun 

机构地区：[1]Center of Advanced Elastomer Materials,College of Materials and Engineering,Beijing University of Chemical Technology,Beijing 100029 [2]College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029 [3]School of Food and Health,Beijing Technology and Business University,Beijing 100048 [4]Institute of Industrial and Consumer Product Safety,Chinese Academy of Inspection and Quarantine,Beijing 100176

出　　处：《China Petroleum Processing & Petrochemical Technology》2020年第2期30-36,共7页中国炼油与石油化工（英文版）

基　　金：the supports of the National Key Research and Development of BioBased Rubber(2017YFB0306900&2017YFB0306901);the National Natural Science Foundation of China(51673012);the Fundamental Research Funds for the Central Universities(PYBZ1828);the Beijing Technology and Business Universtiy Youth Scholoars Funds(PXM2019014213000007)。

摘　　要：Taraxacum kok-saghyz(TKS)is rich in natural rubber(NR),a natural organic macromolecular compound composed of cis-1,4-polyisoprene,and may become the second NR-bearing plant for biochemical engineering development.In this paper,a rapid and quantitative ultra-high performance liquid chromatography tandem mass spectrometry(UHPLCMS/MS)method was established for determination of macromolecular biosynthesis substrate(dimethylallyl pyrophosphate,DMAPP)and initiator(farnesyl pyrophosphate,FPP)contained in TKS.A Kromasil C18 chromatographic column was used for separation,and the multi-reaction monitoring mode(MRM)of triple quadrupole mass spectrometry was used for detection.Quantification was performed by external calibration method.The results showed that the limit of detection(LOD)and the limit of quantitation(LOQ)of DMAPP were 2.42μg/L and 7.26μg/L,respectively,and the LOQ and the LOD of FPP were 1.02μg/L and 3.05μg/L,respectively.At a concentration of 1—1000μg/L,both analytes had good determination coefficients(>0.999)of calibration curve.The recoveries of DMAPP and FPP were between 99.0%and 117.1%.In real samples detection,the contents of DMAPP and FPP in TKS samples were between 23.32—82.77μg/L and 12.03—85.67μg/L,respectively.Thus,this approach is a reliable method to quantify DMAPP and FPP in TKS.

关 键 词：QUANTITATION Taraxacum kok-saghyz(TKS) ISOPRENOIDS ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS) natural rubber(NR) 

分 类 号：O657.63[理学—分析化学] TQ330.32[理学—化学]