神农架林区中蜂蜜理化指标及抗氧化活性分析  

Analysis of Physicochemical Characterization and Antioxidant Activity of Honey Produced by Apis cerana in Shennongjia Forestry District

作　　者：蔡雨娇 孙丽萍[1,2,3,4] 张雪琦 CAI Yu-jiao;SUN Li-ping;ZHANG Xue-qi(Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Key Laboratory of Bee Products for Quality and Safety Control,Ministry of Agriculture and Rural Affairs,Beijing 100093,China;Laboratory of Risk Assessment for Quality and Safety of Bee Products,Ministry of Agriculture and Rural Affairs,Beijing 100093,China;Bee Products Quality Supervision and Testing Center,Ministry of Agriculture and Rural Affairs,Beijing 100093,China;College of Environment and Plant Protection,Hainan University,Haikou 570100,Hainan,China)

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]农业农村部蜂产品质量安全控制重点实验室,北京100093 [3]农业农村部蜂产品质量安全风险评估实验室,北京100093 [4]农业农村部蜂产品质量监督检验测试中心,北京100093 [5]海南大学环境与植物保护学院,海南海口570100

出　　处：《食品研究与开发》2020年第14期183-187,共5页Food Research and Development

基　　金：国家蜂产业技术体系(CARS-45-KXJ10);国家特色农产品风险评估专项(GJFP2019010);中国农业科学院创新工程项目(CAAS-ASTIP-2019-IAR)。

摘　　要：该研究以湖北神农架林区5个中蜂蜜样品为研究对象,对其理化指标和总酚酸、总黄酮含量进行测定,采用DPPH·和ABTS+自由基清除试验测定其抗氧化活性。试验结果表明,神农架林区5个中蜂蜜样品理化指标均符合GH/T 18796-2012《蜂蜜》,总黄酮含量为(9.95±0.04)mg/100 g^(13.66±0.04)mg/100 g;总酚酸含量为(20.69±0.03)mg/100 g^(31.71±0.07)mg/100 g;5个中蜂蜜样品均具有DPPH·和ABTS+自由基清除能力,IC50值分别在(39.14±0.16)mg/mL^(106.63±0.38)mg/mL和(48.48±0.22)mg/mL^(127.07±1.41)mg/mL之间。其中总酚酸含量与清除DPPH·和ABTS+自由基能力存在显著正相关,其相关系数分别为0.973、0.920。该研究首次报道了神农架林区中蜂蜜的理化性质及其抗氧化活性,为神农架林区中蜂蜜的开发利用提供了理论依据。Five honey varieties from Shennongjia forestry district in Hubei were assessed including physical and chemical indicators,contents of total phenolics and total flavonoid.Their antioxidant activities were determined by DPPH·and ABTS+free radical scavenging experiments.The results showed that the physical and chemical indicators of five honey varieties from Shennongjia forestry district were confromed with honey<GH/T 18796-2012>.The contents of total flavonoids ranged from(9.95±0.04)mg/100 g to(13.66±0.04)mg/100 g.The contents of total phenolic acids ranged from(20.69±0.03)mg/100 g to(31.71±0.07)mg/100 g.The five honey varieties were identificated DPPH·and ABTS+free radical scavenging capacity with IC50 of(39.14±0.16)mg/mL to(106.63±0.38)and(48.48±0.22)mg/mL to(127.07±1.41)mg/mL.The total phenolics content was positively correlated with DPPH·and ABTS+free radical scavenging ability,and the correlation coefficients was 0.973 and 0.920,respectively.It was reported the physical and chemical indicators and antioxidant activities of honey in Shennongjia forestry district for the first time in this study,which provided a theoretical basis for the development and utilization of honey in Shennongjia forestry district.

关 键 词：中蜂蜜 总酚酸 总黄酮 自由基清除能力 抗氧化能力 

分 类 号：S896.1[农业科学—特种经济动物饲养]