n-3多不饱和脂肪酸对克罗恩病大鼠炎性因子的影响  

Effects of n-3 polyunsaturated fatty acids on inflammatory factors in rats with Crohn's disease

作　　者：姚嘉茵[1] 麦蕾[2] 丛龙玲[3] 尧新华[3] 鲁义[3] 郅敏[1] 高翔[1] Yao Jiayin;Mai Lei;Cong Longling;Yao Xinhua;Lu Yi;Zhi Min;Gao Xiang(Department of Gastroenterology,Sixth Affiliated Hospital of Sun Yat-Sen University,Guangzhou,Guangdong 510610;Department of Gastroenterology,Fifth Affiliated Hospital of Sun Yat-Sen University,Zhuhai,Guangdong 519000;Department of Anesthesiology,Guangzhou Hospital of Traditional Chinese Medicine,Guangzhou,Guangdong 510130,China)

机构地区：[1]中山大学附属第六医院消化内科,广州510610 [2]中山大学附属第五医院科室消化内科,广东珠海519000 [3]广州市中医医院麻醉科,510130

出　　处：《中华生物医学工程杂志》2019年第6期740-743,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(81900490)。

摘　　要：目的拟进一步明确n-3PUFA对CD大鼠模型免疫平衡的影响。方法采用三硝基苯磺酸(TNBS)诱导建立实验性结肠炎大鼠模型,40只SD大鼠随机分为空白对照组,模型组,n-3PUFA组(20 mg/kg/d灌胃)及强的松组(5 mg/kg/d灌胃)。采用ELISA、RT-PCR及Westernblotting方法检测CD大鼠血清和肠组织的促炎因子(TNF-α、IL-6和IL-17A)以及抑炎因子(IL-10、IL-4和TGF-β)的基因和蛋白表达水平。结果n-3PUFA饮食干预可下调促炎因子表达水平,提高抑炎因子表达水平(P<0.05)。强的松在抑制IL-6表达以及促进TGF-β表达方面优于n-3PUFA(P<0.05)。结论n-3PUFA饮食干预有效维持CD大鼠免疫平衡,其作用与糖皮质激素相仿,这可能是治疗CD的重要作用机制。Objective To investigate the effects of n-3PUFA on the immune balance in rat model of Crohn’s Disease(CD).Methods The rat model of experimental colitis was induced by trinitrobenzenesulfonic acid(TNBS).Forty SD rats were randomly divided into the blank control group,model group,n-3PUFA group(given 20 mg·kg^-1·d^-1 by gavage)and prednisone group(given 5 mg·kg^-1·d^-1 by gavage).ELISA,RT-PCR and Western blotting were used to measure the gene and protein expression levels of pro-inflammatory factors(TNF-α,IL-6 and IL-17A)and anti-inflammatory factors(IL-10,IL-4 and TGF-β)in serum and intestinal tissues of the CD rats.Results Dietary intervention with n-3PUFA diets was shown to down-regulate the expression of pro-inflammatory factors and up-regulate the expression of anti-inflammatory factors(P<0.05).Prednisone appeared more efficient than n-3PUFA in inhibiting the IL-6 expression and promoting the TGF-βexpression(P<0.05).Conclusion Dietary intervention with n-3PUFA may effectively maintain the immune balance in CD rats.Such effect and the possible mechanism are similar as with glucocorticoids.

关 键 词：克罗恩病 N-3多不饱和脂肪酸 糖皮质激素 促炎因子 抑炎因子 

分 类 号：R57[医药卫生—消化系统]