胶体金试纸条同时检测鲤鱼中7种苯并咪唑类药物的残留  

Simultaneous Detection of 7 Benzimidazoles Residues in Carp by Colloidal Gold Immunochromatographic Strip

作　　者：张彩芹 张勋 李洁[1] 李娜 肖香[1] 欧阳琴[1] 王云[1] 周兴华[1] ZHANG Cai-qin;ZHANG Xun;LI Jie;LI Na;XIAO Xiang;OUYANG Qin;WANG Yun;ZHOU Xing-hua(School of Food and Biological Engineering,Jiangsu University,Zhengjiang 212013,China;Jiangsu MeiZheng Biological Technology Co.Ltd.,Wuxi 214000,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏美正生物科技有限公司,江苏无锡214000

出　　处：《现代食品科技》2020年第6期291-296,共6页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2017YFC1600801)。

摘　　要：为检测鲤鱼中苯并咪唑类药物残留,降低其对人体造成的危害,本文基于单克隆抗体之上建立胶体金试纸条检测方法。首先以2-甲氧基羰基氨基-3H-苯并咪唑-5-羧酸为半抗原,用活化酯法偶联蛋白质制备免疫原,免疫小鼠,进行细胞融合制备单克隆抗体,而后用合成的胶体金标记单克隆抗体制备胶体金试纸条。通过间接竞争ELSIA法(ic-ELISA)测得阿苯达唑、甲苯咪唑、阿苯达唑亚砜、阿苯达唑砜、芬苯达唑、氟苯咪唑、奥芬达唑的半抑制浓度(IC50)分别为0.44、0.16、3.47、5.62、0.62、0.10、5.77 ng/mL。胶体金试纸条在鲤鱼中的阿苯达唑、甲苯咪唑、阿苯达唑亚砜、阿苯达唑砜、芬苯达唑、氟苯咪唑、奥芬达唑的检测限分别为4、2、50、100、10、1、100 ng/g,检测时间为15 min。因此,该方法灵敏度高、成本低、速度快且无需仪器辅助,适宜现场大量鲤鱼样本的快速检测。In order to reduce its harm to human,a colloidal gold immunochromatographic strip was established based on monoclonal antibody(mAb)to detect the residue of benzimidazoles in carp.Firstly,the immunogen was prepared using 2-(methoxycarbonylamino)-3H-benzimidazole-5-carboxylic acid as hapten and coupling protein by activated ester method,mice were immunized and mAb was prepared by cell fusion.Then A colloidal gold immunochromatographic strip was prepared by mAb labelled with synthetic colloidal gold.The 50%inhibitory concentrations(IC50)of albendazole,mebendazole,albendazole s-oxide,albendazole sulfone,fenbendazole flubendazole,oxfendazole were 0.44,0.16,3.47,5.62,0.62,0.10,5.77 ng/mL by ic-ELISA,respectively.The limit of detection(LOD)of albendazole,mebendazole,albendazole s-oxide,albendazole sulfone,fenbendazole,flubendazole,oxfendazole were 4,2,50,100,10,1,100 ng/g in carp samples,respectively.Therefore,the developed method was highly sensitive,rapid,low-cost,free-instrument and applicable for on-site detection of a large numbers of carp samples.

关 键 词：苯并咪唑 单克隆抗体 胶体金试纸条 鲤鱼 

分 类 号：S948[农业科学—水产养殖]