腐生葡萄球菌合成纳米钯及其原位催化甲基绿还原  

Synthesis of nanopalladium by Staphylococcus sp. and its in-situ catalytic reduction of methyl green

作　　者：钱怀丽 熊晶晶 邓国志[1,2] 武超[1,2] 程园园[3] QIAN Huaili;XIONG Jingjing;DENG Guozhi;WU Chao;CHENG Yuanyuan(College of Resources and Environmental Engineering,Anhui University,Hefei 230601;Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration,Hefei 230601;School of Life Sciences,Anhui University,Hefei 230601)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]湿地生态保护与修复安徽省重点实验室(安徽大学),合肥230601 [3]安徽大学生命科学学院,合肥230601

出　　处：《安徽农业大学学报》2020年第2期283-287,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(K110138029);安徽省高校自然科学研究项目(Y06050536);安徽省高校优秀青年人才支持计划项目(Y04041820)共同资助。

摘　　要：利用腐生葡萄球菌Staphylococcussp.JJ-^1合成钯纳米颗粒(Bio-Pd),并原位应用于甲基绿的催化还原。通过透射电子显微镜(TEM)、X-射线衍射(XRD)、X射线光电子能谱分析(XPS)和傅里叶红外光谱仪(FTIR)等表征,表明合成了零价钯纳米颗粒,主要分布在细胞外表面,平均直径约为15~40 nm,晶型结构良好。Bio-Pd在1.5h内对甲基绿还原达到99%。通过紫外-可见吸收光谱(UV-visible)、液相色谱-质谱联用(LC-MS)等手段对甲基绿(MG)及还原产物进行分析,产物主要为4-(N,N-二甲基氨基)-4′-(N′,N′-二甲基氨基)二苯甲酮(DDBP),4-(N-乙基-N,N-二甲基氨基)-4′-(N′,N′-二甲基氨基)二苯甲酮(ED-DBP)。结果表明,腐生葡萄球菌Staphylococcus sp. JJ-^1合成钯纳米颗粒并高效催化还原甲基绿。Palladium nanoparticles(bio-Pd) were synthesized by Staphylococcus sp. JJ-^1 and applied to the catalytic reduction of methyl green in situ. Characterization by transmission electron microscopy(TEM),X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR) showed that zero-valent Pd were synthesized. Bio-Pd mainly distributed on the outer surface of cells,and their average diameter was about 15-40 nm with well crystal structure. Synthesized Bio-Pd was used to reduce methyl green and removed 99% of dosed methyl green in 1.5 hours. The products of methyl green reduction were analyzed by ultraviolet-visible absorption spectroscopy(UV-visible) and liquid chromatography-mass spectrometry(LC-MS). Main products were 4-(N,N-dimethylamino)-4’-(N’,N’-dimethyl Amino) benzophenone(DDBP) and 4-(N-ethyl-N,N-dimethylamino)-4’-(N’,N’-dimethylamino)benzophenone(ED-DBP).The results showed that Staphylococcus sp. JJ-^1 synthesized palladium nanoparticles and efficiently reduced methyl green.

关 键 词：腐生葡萄球菌 纳米钯 催化还原 甲基绿 

分 类 号：X172[环境科学与工程—环境科学]