拟南芥CRISPR/Cas9基因编辑虚拟仿真实验系统建设  

Construction of virtual simulation system of CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

作　　者：霍颖异[1,2] 王君晖[2] 史影[1,2] 王国强 吴敏[1,2] HUO Yingyi;WANG Junhui;SHI Ying;WANG Guoqiang;WU Min(National Experimental Teaching Demonstration Center for Biology Education,Zhejiang University,Hangzhou 310058,China;College of Life Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学国家级生物实验教学示范中心,浙江杭州310058 [2]浙江大学生命科学学院,浙江杭州310058

出　　处：《实验技术与管理》2020年第6期137-140,共4页Experimental Technology and Management

基　　金：浙江省“十三五”省级重点建设实验教学示范中心项目;浙江大学2019年度虚拟仿真实验教学培育项目。

摘　　要：结合实验教学中实际经验和课程需求,利用学科优势,依托前沿科研成果,设计并建设了拟南芥CRISPR/Cas9基因编辑虚拟仿真实验系统,将虚拟仿真技术应用于遗传学实验教学。通过该实验系统,学生不仅可以自主学习并掌握基因编辑的相关理论知识和全过程实验操作,有效激发学习兴趣,还可以通过反复练习并配合实体实验教学,切实提高实践能力。该实验开拓了遗传学虚拟仿真实验新资源和实验教学新途径。Based on the experience in experimental teaching practice and curriculum requirements,by taking the advantage of the discipline and relying on the scientific research achievements,the virtual simulation technology is applied to the genetics experiment teaching.A virtual simulation experiment system,i.e.,CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana,is developed.Through this virtual experiment system,students can learn the relevant theoretical knowledge and the entire process of experimental operation independently,which effectively stimulates their interest and curiosity.The system can also improve their practical ability through repeatedly practicing the operation online along with traditional bench-top experiment offline.This system,as a new resource of genetic virtual simulation experiment,forms a new way of genetic experiment teaching.

关 键 词：虚拟仿真实验 实验教学 基因编辑 CRISPR/Cas9 

分 类 号：Q-33[生物学] TP391.9[自动化与计算机技术—计算机应用技术]