Genistein and daidzein reduced chlorpyrifos induced damage in PC12 cell  

作　　者：Yu Gao Jiajia Xu Menglei Xu Sunsen Shi Jinfeng Xiong 

机构地区：[1]College of Plant Protection,Jilin Agricultural University,Changchun,China [2]College of Food Science and Engineering/State Key Laboratory of Supramolecular Structure and Materials,Jilin University,Changchun,China [3]Changchun Institute of Biological Products,Changchun,China

出　　处：《Oil Crop Science》2020年第1期1-5,共5页中国油料作物学报(英文版）

基　　金：supported by the National Key Technology R&D Program of the Ministry of Science and Technology(2012BAD33B03);China Agriculture Research System(CARS-04);the China Postdoctoral Science Foundation(2018M631876);Open Research Fund of National Research Center of Engineering and Technology of Tea Quality and Safety(2017NTQS0201)。

摘　　要：The present study preliminarily evaluated neurotoxicity injuries induced by chlorpyrifos in PC12 cell,which were used as a model for nervous cell system.In cultured PC12 cell,application of soy isoflavones(genistein,daidzein,monomer and mixture)significantly reduced chlorpyrifos induced toxicity,a widely used pesticide,and resulted in a better cell survival rate.Treatments with isoflavones reduced malondialdehyde content,reactive oxygengeneration and acetylcholine level in medium,and maintained mitochondrial membrane potential integrity.Daidzein enhanced endogenous antioxidant system in PC12 cell with an increasing in superoxide dismutase perunit activity.Genistein reduced acetylcholine content in the medium.Daidzein and genistein showed different effects,and their combined effect were greater than individual.In conclusion,soy isoflavones as an antioxidant and neuroprotectant,enhanced choline metabolism,which effectively mitigated disadvantageous influence of PC12 cell caused by chlorpyrifos.

关 键 词：CHLORPYRIFOS Soy isoflavone GENISTEIN DAIDZEIN PC12 cell CYTOTOXICITY 

分 类 号：R99[医药卫生—毒理学]