Elevated methionine content in soybean seed by overexpressing maizeβ-zein protein  

作　　者：Chen Guo Xiujie Liu Li Chen Yupeng Cai Weiwei Yao Shan Yuan Cunxiang Wu Tianfu Han Shi Sun Wensheng Hou 

机构地区：[1]National Center for Transgenic Research in Plants,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,China [2]Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《Oil Crop Science》2020年第1期11-16,共6页中国油料作物学报(英文版）

基　　金：supported by the Ministry of Science and Technology of China(2016YFD0100504);National Natural Science Foundation of China(31471571);Major Science and Technology Projects of China(2016ZX08004-003,2016ZX08010-004);CAAS(Chinese Academy of Agriculture Sciences)Agricultural Science and Technology Innovation Project。

摘　　要：Soybean provides superior and readily available protein for human and livestock.However,nutritional value of soybean is limited due to the deficiency of an essential amino acid,methionine.To improve total methionine content of soybean,a methionine-rich seed storage protein,β-zein,was introduced into soybean cultivar’Jack’under the control of legumin B4 promoter or Ca MV 35S promoter.Totally 4 T3transgenic lines exhibited higher expression levels of foreign genes,and legumin B4 promoter directed a stronger accumulation ofβ-zein protein than Ca MV 35S promoter.Compared to wild type plant,total methionine content in transgenic soybean seeds significantly increased by up to approximately 15%.Although the introduction ofβ-zein gene improved total methionine content,the level was negligible compared to native soybean storage proteins,implying that the inadequate soluble methionine is the limiting factor.Based on these observations,a new strategy for simultaneously increasing the"source"and"sink"of methionine metabolism is proposed to further improvement of total methionine content in soybean seed.

关 键 词：SOYBEAN PROTEIN METHIONINE β-Zein NUTRITION 

分 类 号：S565.1[农业科学—作物学]