茶多酚对高尿酸血症小鼠尿酸水平及肝肾组织的影响  

Effect of Tea Polyphenols on Uric Acid Level,Liver and Kidney Tissue of Hyperuricemia Mice

作　　者：任群利 廖成成 刘建国 范芹 王苗 王倩 汪蓓蕾 胡欢 REN Qun-li;LIAO Cheng-cheng;LIU Jian-guo;FAN Qin;WANG Miao;WANG Qian;WANG Bei-lei;HU Huan(Institute of Life Sciences,Zunyi Medical University,Special Key Laboratory of Microbial Resources and Drug Development,Higher Education Institution in Guizhou Province,Zunyi Guizhou 563000;Institute of Life Sciences,Zunyi Medical University,Special Key Laboratory of Oral Diseases Research,Higher Education Institutions in Guizhou Province;Stomatology College of Zunyi Medical University)

机构地区：[1]遵义医科大学生命科学研究院贵州省普通高等学校微生物资源与药物开发特色实验室,贵州遵义563000 [2]遵义医科大学生命科学研究院贵州省普通高等学校口腔疾病研究特色重点实验室 [3]遵义医科大学口腔医学院

出　　处：《现代农业科技》2020年第13期200-202,206,共4页Modern Agricultural Science and Technology

基　　金：遵义医学院硕士启动基金项目(F-904);遵义医学院优秀青年人才项目(17zy-002);国家自然科学基金委地区基金项目(31860076);遵义市科技局基金项目[2018(29)]。

摘　　要：本文以雄性昆明小鼠为试验动物,通过比较小鼠血清中尿酸、尿素氮、肌酐的含量,检测肝肾和血清中的黄嘌呤氧化酶,以及观察肝肾组织病理特征,研究茶多酚对氧嗪酸钾诱导的小鼠高尿酸血症的治疗作用。结果表明,与模型组相比,茶多酚高剂量组(600 mg/kg)高尿酸血症小鼠血清中尿酸含量极显著减少约33.7%,茶多酚中剂量(300 mg/kg)和低剂量组(150 mg/kg)均极显著减少约28.5%,别嘌醇组(10 mg/kg)极显著减少99.5%(P<0.01)。茶多酚中、高剂量组能降低血清尿素氮、肌酐含量;同时,肝、肾中黄嘌呤氧化酶活性分别极显著降低50%、64%(P<0.01)。病理组织形态学检查结果发现,别嘌醇组小鼠肝脏有一定损伤,肾脏有中度病理组织学改变;模型组和茶多酚各剂量组小鼠肝脏损伤影响不大,仅有轻度水肿;模型组小鼠肾脏损伤较轻,茶多酚低剂量组可见小鼠肾脏轻度病理组织学改变,茶多酚中、高剂量组未见明显变化。由此可知,茶多酚能显著降低氧嗪酸钾诱导的高尿酸血症小鼠血尿酸水平,可能与抑制尿酸的生成和促进排泄相关。同时,茶多酚对小鼠肝肾组织具有一定的保护作用。The male Kunming mice were used as experimental animals in this paper,the therapeutic effect of tea polyphenols on hyperuricemia mice induced by potassium oxyzinate by comparing the serum uric acid,urea nitrogen and creatinine contents of the mice,detecting the xanthine oxidase in liver,kidney and serum,and observing the pathological features of liver and kidney tissue was studied.The results showed that compared with model group,the content of serum uric acid in tea polyphenols high dose group(600 mg/kg)significantly decreased by about 33.7%,in the tea polyphenols middle dose group(300 mg/kg)and low dose group(150 mg/kg)all significantly decreased by about 28.5%,in the allopurinol group(10 mg/kg)significantly decreased by 99.5%(P<0.01).The contents of serum urea nitrogen and creatinine decreased in tea polyphenols middle and high dose groups;and the activities of xanthine oxidase in liver and kidney were significantly reduced by 50%and 64%(P<0.01),respectively.Pathomorphological exami-nation showed that the liver was damaged to a certain extent in allopurinol group,the kidney had moderate pathological changes;the liver damage was not significant in model group and tea polyphenol groups,with slight edema;the kidney injury was slight in model group,slight histopathological changes were observed in tea polyphenols low dose group,while no significant changes were observed in tea polyphenols middle and high dose groups.Therefore,tea polyphenols can significantly reduce the serum uric acid level of hyperuricemia mice induced by potassium oxyzinate,it may be associated with inhibiting the generation and promoting the excretion of uric acid.At the same time,tea polyphenols has a certain protective effect on the liver and kidney tissue of mice.

关 键 词：茶多酚 小鼠 尿酸 高尿酸模型 病理组织 

分 类 号：R151[医药卫生—营养与食品卫生学]