MALDI-TOF-MS技术在块菌、美味牛肝菌物种快速甄别中的运用  

Application of MALDI-TOF-MS Technology in Identificationof Tuber indicum and Boletus bainiugan

作　　者：陈丽萍 闫占鹏 赖梦书 陈芸 李芳 吕廷保 范效英 刘燕 丁元明 CHEN Li-ping;YAN Zhan-peng;LAI Meng-shu;CHEN-Yun;LI Fang;LYU Ting-bao;FAN Xiao-ying;LIU Yan;DING Yuan-ming(Technical Center of Kunming Customs District,Kunming 650200,China;Autobio Labtec Instruments Co.Ltd,Zhengzhou 450001,China;Hekou Customs Comprehensive Technical Service Center,Hekou 661300,China)

机构地区：[1]昆明海关技术中心,云南昆明650200 [2]安图实验仪器(郑州)有限公司,河南郑州450001 [3]河口海关综合技术服务中心,云南河口661300

出　　处：《中国食用菌》2020年第5期37-42,共6页Edible Fungi of China

基　　金：“十三五”国家重点研发计划项目(2017YFF0211300);“十三五”国家重点研发计划项目(2017YFF0211301)。

摘　　要：运用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS),对2种云南特色高值野生食用菌块菌和美味牛肝菌进行物种甄别鉴定。运用液氮甲酸法处理样品,获得浓度分别为0.243 mg·mL^-1和0.284 mg·mL^-1的块菌和美味牛肝菌的蛋白质提取液,经MALDI-TOF-MS采集指纹图谱,对块菌和美味牛肝菌的蛋白指纹图谱进行比对与聚类分析。结果表明,产地不同的同种野生食用菌具有相似的蛋白指纹图谱和物种特征质谱峰,而不同种类的食用菌间具有差异明显的特征蛋白指纹图谱。MALDI-TOF-MS可以用于云南出口高值特色野生食用菌块菌和美味牛肝菌的物种快速甄别。Two edible fungi of Tuber indicum Cooke&Massee and Boletus bainiugan Dentinger were classified and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry MALDI-TOF-MS technology.The samples were treated with liquid nitrogen formic acid,and the protein extracts of T.indicum and B.bainiugan were obtained with concentrations of 0.243 mg·mL^-1 and 0.284 mg·mL^-1,respectively.The protein fingerprints of T.indicum and B.bainiugan were compared and cluster analyzed by MALDI-TOF-MS.The results showed that the same kind of wild edible fungi from different areas had similar protein fingerprints and species characteristic mass spectrum peaks,but different kinds of edible fungi had different characteristic protein fingerprints.MALDI-TOF-MS can be used to identify the high value species of T.indicum and B.bainiugan in Yunnan province.

关 键 词：块菌 美味牛肝菌 身份甄别 蛋白指纹图谱 MALDI-TOF-MS 

分 类 号：S646.9[农业科学—蔬菜学]