Chlorella vulgaris胞内多糖抗氧化活性及其与糖代谢相关酶的关系  

Assessing the antioxidant activity of intracellular polysaccharide from Chlorella vulgaris and its relationship with glycometabolism-related enzymes

作　　者：孙建瑞[1] 赵君峰[1] 符丹丹[1] 张彬[1] 古绍彬[1] 王大红[1] SUN Jianrui;ZHAO Junfeng;FU Dandan;ZHANG Bin;GU Shaobin;WANG Dahong(College of Food and Bioengineering,Henan Engineering Research Center of Food Microbiology,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南省食品微生物工程技术研究中心,洛阳471023

出　　处：《应用与环境生物学报》2020年第3期512-519,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31401672,31701536);河南省高等学校重点科研项目(17B180003)资助。

摘　　要：对淡水微藻Chlorella vulgaris 224胞内多糖的抗氧化活性进行研究,并对其在不同培养条件下生长发育过程中胞内多糖的积累与糖代谢相关酶的关系进行分析.C.vulgaris 224胞内多糖浓度为60 mg/mL时其对DPPH自由基的清除率为61.62%,浓度为30 mg/mL时其对羟基自由基的清除率超过50%,结果表明其胞内多糖具有较强的抗氧化活性.低盐条件下己糖激酶、苹果酸脱氢酶、6-磷酸葡萄糖脱氢酶和磷酸葡萄糖异构酶的活性均高于正常试验组;低氮条件下己糖激酶、苹果酸脱氢酶、6-磷酸葡萄糖脱氢酶的活性均低于正常试验组,而磷酸葡萄糖异构酶的活性高于正常组;添加NaHCO_3时己糖激酶、苹果酸脱氢酶、6-磷酸葡萄糖脱氢酶的活性均低于正常组,而磷酸葡萄糖异构酶的活性高于正常组;相关性分析发现己糖激酶、苹果酸脱氢酶和6-磷酸葡萄糖脱氢酶与其胞内多糖的积累呈显著相关(P<0.05),而磷酸葡萄糖异构酶与胞内多糖积累的相关性未到达显著水平(P>0.05).本研究表明己糖激酶、苹果酸脱氢酶和6-磷酸葡萄糖脱氢酶是调控C.vulgaris 224胞内多糖积累的关键酶,结果可为筛选天然抗氧化物质提供一定理论基础.In this study,the antioxidant activity of intracellular polysaccharide derived from Chlorella vulgaris 224 was assessed.Thereafter,the relationship between accumulation of intracellular polysaccharide and glycometabolism-related enzymes during the growth and development of C.vulgaris 224 under different culture conditions was analyzed.The clearance rate of DPPH free radical was estimated as 61.62%when the concentration of intracellular polysaccharide was 60 mg/mL,and the clearance rate of hydroxyl radical exceeded 50%when the concentration of intracellular polysaccharide was 30 mg/mL.The activities of HK,MDH,G6PDH,and PGI under low salt condition were higher than those in normal condition.The activities of HK,MDH,and G6PDH under low nitrogen condition were lower than that in normal condition,while the activity of PGI was higher than that in normal condition.The activities of HK,MDH,and G6PDH were lower than that in normal condition when NaHCO3 was added,while the activity of PGI was higher than that in normal condition.Results of correlation analysis revealed that HK,G6PDH,and MDH were significantly correlation(P<0.05)with intracellular polysaccharide accumulation,but the correlation between PGI and intracellular polysaccharide accumulation was not significant(P>0.05).These results indicate that the intracellular polysaccharide of C.vulgaris 224 exhibits strong antioxidant activity.In addition,we believe that HK,G6PDH,and MDH are the key enzymes regulating the accumulation of intracellular polysaccharide in C.vulgaris 224.

关 键 词：小球藻 胞内多糖 抗氧化活性 糖代谢相关酶 

分 类 号：Q946.5[生物学—植物学]