油菜蜂花粉蛋白酶法破壁提取及结构表征  

Enzymatic breaking-extraction and characterizationof protein from rape bee pollen

作　　者：王凌波 朱松[1,2] 陈尚卫[1,2] WANG Ling-bo;ZHU Song;CHEN Shang-wei(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与机械》2020年第6期151-156,共6页Food and Machinery

基　　金：国家自然科学基金面上项目(编号:31871794)。

摘　　要：以油菜蜂花粉为原料,研究酶法破壁提取蛋白的效果,并对蜂花粉蛋白进行纯化和表征。先对蜂花粉蛋白的提取条件进行单因素及响应面优化,以蛋白提取率为指标,确定最佳提取条件为:将2.5 g峰花粉和50 mL pH 5.96的水溶液混合后,加入体积分数0.4%的复合植物水解酶(100 FBG/g),42℃振荡酶解6.5 h,该条件下蛋白提取率为79.53%。蜂花粉提取液经Sephadex G-100凝胶柱分离后共得到4个组分,经测定其中仅一个组分中含有蛋白质,含量为66.77 g/100 g;疏水性氨基酸含量为27.52 g/100 g,氨基酸组成分析和红外光谱结果都表明蜂花粉蛋白中存在芳香族氨基酸;圆二色谱结果表明蜂花粉蛋白易被酶解。The bee pollen of oilseed rape was used to investigate the effect of enzymatically breaking the wall for protein extraction,and the optimal conditions of the extraction were determined by single factor test and response surface analysis.After mixing 2.5 g of peak pollen and 50 mL of pH 5.96 aqueous solution,0.4%volume fraction compound plant invertase(100 FBG/g)was added and then stirring and extracting at 42℃for 6.5 h.Under the control of these optimal conditions,the protein pollen extracts were separated by Sephadex G-100 gel column to obtain a total of 4 components.However,only one of them was determined to contain protein with 66.77 g/100 g,with the contain of hydrophobic amino acid of 27.52 g/100 g.The amino acid composition analysis and infrared spectroscopy results showed that aromatic amino acids were found in bee pollen protein.Moreover,the circular dichroism chromatography results showed that the proteins in bee pollen were easily digested enzymatically for producing peptides.

关 键 词：蜂花粉 蛋白 酶法破壁 提取率 结构表征 

分 类 号：TS201.21[轻工技术与工程—食品科学]