地果的转录组学分析  

Transcriptome Analysis of Chinese Ficus tikoua

作　　者：石登红 王继玥 白禹 张婷 刘燕 欧阳柏成[2] SHI Deng-hong;WANG Ji-yue;BAI Yu;ZHANG Ting;LIU Yan;OUYANG Bo-cheng(School of Biological and Environmental Engineering,Guiyang University,Guiyang 550005,China;School of Mathematics and Information Engineering,Guiyang University,Guiyang 550005,China;Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region,Guiyang 550005,China)

机构地区：[1]贵阳学院生物与环境工程学院,贵阳550005 [2]贵阳学院数学与信息工程学院,贵阳550005 [3]贵州省山地珍稀动物与经济昆虫重点实验室,贵阳550005

出　　处：《热带亚热带植物学报》2020年第4期317-328,共12页Journal of Tropical and Subtropical Botany

基　　金：This work was supported by the Project of China Scholarship Council(Grant No.LJF[2017]50863);the Program for National Innovation Training of Undergraduate(Grant No.2018109760007,2018109760008);the Program for First-class Discipline Construction in Guizhou Province(Grant No.QJKYF[2017]85);the Project for Science and Technology of Guizhou Province(Grant No.QKHJC[2017]1004);the Project for Major Research by Innovation Group in Guizhou Education Department(Grant No.QJHKY[2018]027);the Project for Provincial Key Discipline of Ecology(Grant No.ZDXK[2013]08);the Project for Cooperative Base for Production,Education and Research of Organic Vegetable Ecological Cultivation and Breeding(Grant No.KY[2013]125);the Program for Innovative Training of Undergraduate in Guizhou(Grant No.2018520777,2018520778,2018520831,2018520861,20195200341,20195201373);the Program for Educational Incubation of Guiyang University(Grant No.GYU-KJT[2019]-06,GYU-KJT[2019]-07);the Discipline and Master's Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University(Grant No.SH-2020).

摘　　要：为探究地果(Ficus tikoua)的遗传变异特征,利用Illumina HiSeq-2500平台获取地果的基因表达谱。结果表明,共获得了197362个转录本,总长度和平均长度分别为114072125和577 bp。使用BlastX和BlastN共注释了139992个转录本(占总数的70.93%)。从3个品种叶片和茎的6对样品中,分别鉴定了12397、12340、10373、94431、71830和44465个差异表达基因,以及注释了126、129、125、134、138和137条代谢途径。这将有助于理解地果的遗传特征,以及不同组织中代谢途径的变化。Ficus tikoua is a woody plant used as traditional medicine.To investigate the genetic variation of F.tikoua cultivars,the gene expression profiles of Chinese F.tikoua were obtained by using the Illumina HiSeq 2500 platform.The results showed that a total of 197362 transcripts were obtained from the transcriptome of F.tikoua,with a total and average length of 114072125 and 577 bp,respectively.There were 139992 transcripts(70.93%of total transcripts)annotated by using BlastX and BlastN.There were 12397,12340,10373,94431,71830,and 44465 differentially expressed genes identified,and 126,129,125,134,138,and 137 metabolic pathways annotated from six pairs of tissue samples,blades and stems of three F.tikoua cultivars,respectively.So,these would help for understanding not only the genetic characteristics of F.tikoua,but also the metabolic pathway changes in different tissues.

关 键 词：转录组 地果 RNA-SEQ 差异表达基因 

分 类 号：S567.19[农业科学—中草药栽培]